This study aimed to examine the effect of follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), and growth hormone (GH) on Aquaporin 5 (AQP5) expression in granulosa (Gc) and theca cells (Tc) from medium (MF) and large (LF) ovarian follicles of pigs. The results showed that GH significantly decreased the expression of AQP5 in Gc from MF in relation to the control. In the Gc of large follicles, PRL stimulated the expression of AQP5. However, the increased expression of AQP5 in the Tc of LF was indicated by GH and PRL in relation to the control. A significantly higher expression of the AQP5 protein in the Gc from MF and LF was indicated by FSH and PRL. In co-cultures, an increased expression of AQP5 was observed in the Gc from LF incubated with LH, PRL, and GH. A significantly increased expression of AQP5 was also observed in co-cultures of Tc from all type of follicles incubated with LH, whereas PRL stimulated the expression of AQP5 in Tc from MF. Moreover, AQP5 protein expression increased in the co-culture isolated from MF and LF after treatment with FSH, LH, PRL, and GH. AQP5 immunoreactivity was observed in the cytoplasm, mainly in the perinuclear region and endosomes, as well as in the cell membranes of Gc and Tc from the LF and MF.

• Klíčová slova
• Aquaporin 5, gene and protein expression, in vitro study, ovarian cells, pig,
• MeSH
• akvaporin 5 genetika   MeSH
• biologické markery MeSH
• folikulární buňky účinky léků   metabolismus   MeSH
• folikuly stimulující hormon metabolismus   MeSH
• hypofyzární hormony metabolismus   farmakologie   MeSH
• kokultivační techniky MeSH
• luteinizační hormon metabolismus   MeSH
• ovariální folikul cytologie   účinky léků   metabolismus   MeSH
• prasata MeSH
• prolaktin metabolismus   MeSH
• regulace genové exprese u rostlin * MeSH
• růstový hormon metabolismus   MeSH
• thekální buňky účinky léků   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• akvaporin 5 MeSH
• biologické markery MeSH
• folikuly stimulující hormon MeSH
• hypofyzární hormony MeSH
• luteinizační hormon MeSH
• prolaktin MeSH
• růstový hormon MeSH

The aim of the study was to determine the expression of proliferating cell nuclear antigen protein (PCNA) in the pig ovary. The localization of PCNA was demonstrated in paraffin sections of pig ovarian tissue using primary mouse monoclonal anti-PCNA antibody. In primordial follicles, no remarkable staining for PCNA either in granulosa cells or in the oocytes was observed. In primary to secondary follicles, positive staining in oocytes and in some granulosa cells was detected. The advanced preantral and particularly actively growing small to large antral follicles showed extensive PCNA labeling in the layers of granulosa and theca cells and in the cumulus cells encircling the oocyte. PCNA labeling was expressed in nuclei of oocytes in preantral and small antral follicles. In atretic follicles, the level of PCNA protein expression was dependent on the stage of atresia. Follicles demonstrating advanced atresia showed only limited or no PCNA labeled granulosa and theca cells. The results of the study demonstrate that follicular growth and development in pig ovary may be effectively monitored by determining the granulosa cell expression of PCNA.

• MeSH
• buněčné jádro metabolismus   MeSH
• folikulární buňky cytologie   metabolismus   MeSH
• imunohistochemie MeSH
• oocyty cytologie   metabolismus   MeSH
• prasata MeSH
• proliferace buněk * MeSH
• proliferační antigen buněčného jádra biosyntéza   MeSH
• regulace genové exprese fyziologie   MeSH
• thekální buňky cytologie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• proliferační antigen buněčného jádra MeSH

• MeSH
• antigeny bakteriální imunologie   MeSH
• Borrelia imunologie   izolace a purifikace   MeSH
• imunoblotting metody   MeSH
• lidé MeSH
• lymská neuroborelióza diagnóza   imunologie   MeSH
• prediktivní hodnota testů MeSH
• protilátky bakteriální analýza   MeSH
• specificita protilátek MeSH
• thekální buňky imunologie   MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• srovnávací studie MeSH
• Názvy látek
• antigeny bakteriální MeSH
• protilátky bakteriální MeSH

According to available literary data, an increased nuclear volume of endocrine gland cells, various structures of the diencephalon and endocrine ovarial structures could be observed when the respective organs and structures were more active; a decreased nuclear volume was revealed when the activity of the respective organ or its structure was suppressed (Mess, 1962; Maracek and Arendarcik, 1976, 1978). Attention was paid to the karyometric analysis of follicular cells of the stratum granulosum (SG) and secretory cells of the theca folliculi interna (TFI) during selection of the dominant ovulatory follicle (DOF). The aim of this study was to determine differences in hte nuclear volumes of the granulosa follicular cells and the secretory cells of the inner theca of the DO1F in comparison with the largest atretic follicles (AF) as well as to verify the use of karyometric variance analysis for the evaluation of the selection process of the dominant ovulatory tertiary follicle. Histological sections were prepared from large DOF and AF from the ovaries of 18 sheep aged 3-5 years and held under standard conditions of a controlled herd. Control group I (n = 3) consisted of Wallachian sheep on day 15 of the sexual cycle (day 0). Excisions of ovaries from the animals of the experimental group (n = 3) were made on day 16 of the cycle after i.m. treatment with 120 micrograms GnRH 10 and 7 hours prior to sampling. Control group II (n = 3) consisted of Tsigai sheep at the 9th or 10th day of the oestrous cycle (hour 0) and three experimental groups (n = 3 each) consisted of Tsigai sheep 24, 48 and 72 hours after i.m. treatment with 125 micrograms cloprostenol. After excision the ovaries were fixed in neutral formaline, cut into 4 mm thick transverse segments and subjected to standard histological processing. After staining with Harris' HE the 5-7 microns thick sections were karyometrically evaluated at a magnification of 2200 x according to the method of Palkovits (1961) using the PC programme Karyotest 03 (Maracek et al., 1991). 200 cells were evaluated from each sample and both cell types; altogether 7200 cells were examined. GnRH treatment (Dirigestran inj. Spofa) increased the nuclear volume of follicular cells (SG) in the dominant ovulatory follicle in the process of selection (Tab. I, Fig. 1). The former, however, reduced the nuclear volume not only of the SG follicular cells of LAF (Tab. II, Fig. 2) but also of the secretory cells of both the DOF and AF theca interna (Tab. II, Figs. 3, 4). Cloprostenol treatment (Oestrophan inj. Spofa) affected the follicular cells of the granulosa in the dominant ovulatory follicle.(ABSTRACT TRUNCATED AT 400 WORDS)

• MeSH
• folikulární atrézie MeSH
• folikulární buňky ultrastruktura   MeSH
• karyometrie * MeSH
• ovariální folikul fyziologie   ultrastruktura   MeSH
• ovce anatomie a histologie   MeSH
• thekální buňky ultrastruktura   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH

• MeSH
• druhová specificita MeSH
• ovariální folikul růst a vývoj   MeSH
• ovarium anatomie a histologie   MeSH
• thekální buňky MeSH
• želvy anatomie a histologie   MeSH
• zona pellucida MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Indie MeSH

• MeSH
• lidé MeSH
• menstruace MeSH
• ovariální folikul anatomie a histologie   ultrastruktura   MeSH
• thekální buňky klasifikace   ultrastruktura   MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH