The expression of Ruminococcus flavefaciens 007S cellulases in different incubation time points (growth stages) and their substrate inducibility were analyzed by comparing the zymogram expression profiles of cultures grown on insoluble cellulose (Avicel) with cellobiose-grown cultures. The molecular weights of the enzymes were compared to (putative) cellulases encoded in the R. flavefaciens FD-1 genome.

• MeSH
• bakteriální proteiny chemie   genetika   metabolismus   MeSH
• celobiosa metabolismus   MeSH
• celulasy chemie   genetika   metabolismus   MeSH
• celulosa metabolismus   MeSH
• enzymatické testy MeSH
• exprese genu MeSH
• molekulární sekvence - údaje MeSH
• molekulová hmotnost MeSH
• Ruminococcus chemie   enzymologie   genetika   růst a vývoj   MeSH
• terciární struktura proteinů MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• bakteriální proteiny MeSH
• celobiosa MeSH
• celulasy MeSH
• celulosa MeSH

The addition of 100-300 mmol/L of acetic, propionic, butyric or lactic acids (short-chain acids), or of acetic, propionic, and butyric acids (volatile fatty acids, VFA) mixtures increased the degradation of carboxymethyl cellulose (CMC) by R. albus (7.5 to 46 and 6 to 39 %, respectively). Differences among individual acids were observed at 300 mmol/L whereas VFA mixtures differed at 100 mmol/L. When assayed at the same concentration, CMCase activity was increased less by NaCl than by the short-chain acids, whereas ethylene glycol decreased the activity. Since osmolarity and/or ionic strength changes in the medium cannot completely account for the observed increases of carboxymethylcellulase (CMCase) activity, it is suggested that the anions of short-chain acids produce changes in the reaction media polarity that contribute to the effects observed. Alterations in the media could also bring about conformational changes in CMCase leading to increased rates of reaction and subsequent increases in CMC degradation. Finally, explanations for the observed phenomena based on the direct effect of the compounds tested on the cellulosome complex, its domains, and/or its component enzymes are proposed.

• MeSH
• acetáty farmakologie   MeSH
• bachor mikrobiologie   MeSH
• butyráty farmakologie   MeSH
• celulasa metabolismus   MeSH
• chlorid sodný farmakologie   MeSH
• propionáty farmakologie   MeSH
• Ruminococcus enzymologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• acetáty MeSH
• butyráty MeSH
• carboxymethylcellulase MeSH Prohlížeč
• celulasa MeSH
• chlorid sodný MeSH
• propionáty MeSH