Bio-nano interactions have been extensively explored in nanomedicine to develop selective delivery strategies and reduce systemic toxicity. To enhance the delivery of nanocarriers to cancer cells and improve the therapeutic efficiency, different nanomaterials have been developed. However, the limited clinical translation of nanoparticle-based therapies, largely due to issues associated with poor targeting, requires a deeper understanding of the biological phenomena underlying cell-nanoparticle interactions. In this context, we investigate the molecular and cellular mechanobiology parameters that control such interactions. We demonstrate that the pharmacological inhibition or the genetic ablation of the key mechanosensitive component of the Hippo pathway, i.e., yes-associated protein, enhances nanoparticle internalization by 1.5-fold. Importantly, this phenomenon occurs independently of nanoparticle properties, such as size, or cell properties such as surface area and stiffness. Our study reveals that the internalization of nanoparticles in target cells can be controlled by modulating cell mechanosensing pathways, potentially enhancing nanotherapy specificity.
- Klíčová slova
- bio−nano interactions, mechanobiology, mechanotransduction, nanoparticles,
- MeSH
- adaptorové proteiny signální transdukční metabolismus MeSH
- buněčný převod mechanických signálů účinky léků MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- nanočástice * chemie MeSH
- nanomedicína MeSH
- signální dráha Hippo MeSH
- signální proteiny YAP MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- adaptorové proteiny signální transdukční MeSH
- signální proteiny YAP MeSH
- YAP1 protein, human MeSH Prohlížeč
