The bodies of primary sensory neurons and their satellite glial cells (SGCs) are limited by the basal laminae from extracellular matrix of the dorsal root ganglia (DRG). The basal laminae displayed uniform immunofluorescence staining for laminin-1 in the sections of rat intact (naive) DRG. A proximal or distal ligature of the spinal nerves resulted in a heterogeneous immunostaining for laminin-1 around neuron-SGC units in the sections of the corresponding DRG. The pattern of irregular laminin-1 immunofluorescence was more extensive in the ipsilateral than the contralateral DRG of the operated rats. The immunofluorescence for laminin-1 exactly coincided with binding of Concanavalin-A as well as immunostaining for type IV collagen in both naive DRG and DRG affected by nerve ligature. Nidogen immunostaining decreased or fully disappeared at the surface of the SGCs consistently with immunofluorescence staining for laminin-1, but retained or increased in the endothelial cells and ED-1 positive cells invaded the DRG affected by nerve ligature. The results indicate an alteration of the content of basal laminae surrounding the bodies of primary sensory neurons and their SGSs following nerve constriction injury. A modulation of the basal laminae may be related with other cellular and molecular alterations related with peripheral neuropathic pain, for example, expansion of sympathetic sprouts.

• MeSH
• barvení a značení metody   MeSH
• bazální membrána chemie   MeSH
• ektodysplasiny analýza   MeSH
• fluorescenční protilátková technika metody   MeSH
• kolagen typu IV analýza   MeSH
• konkanavalin A analýza   MeSH
• krysa rodu Rattus MeSH
• laminin analýza   MeSH
• membránové glykoproteiny analýza   MeSH
• neuroglie chemie   patologie   MeSH
• potkani Wistar MeSH
• spinální ganglia chemie   zranění   patologie   MeSH
• stenóza MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• ektodysplasiny MeSH
• kolagen typu IV MeSH
• konkanavalin A MeSH
• laminin 1 MeSH Prohlížeč
• laminin MeSH
• membránové glykoproteiny MeSH
• nidogen MeSH Prohlížeč