OBJECTIVES: A simple, cost-effective, and fast gas chromatography method with mass spectrometry detection (GC-MS) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicology purposes. DESIGN AND METHODS: Successful derivatization of glycolic acid with isobutyl chloroformate was achieved directly in serum/urine while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min. RESULTS: The assay was proved to be quadratic in the range of 50 to 5000 mgL(-1) with adequate accuracy (96.3-105.8%) and precision (CV ≤ 8.9%). CONCLUSION: The method was successfully applied to quantify the selected compounds in serum of patients from emergency units and the results correlated well with parallel GC-FID measurements (R(2) 0.9933 for ethylene glycol and 0.9943 for glycolic acid).

• Klíčová slova
• Chloroformate, GC-MS, Glycolic acid, Glycols, Toxicology,
• MeSH
• analýza nákladů a výnosů * MeSH
• časové faktory MeSH
• ethylenglykol krev   moč   MeSH
• glykoláty krev   moč   MeSH
• lidé MeSH
• náhlé příhody MeSH
• plynová chromatografie s hmotnostně spektrometrickou detekcí ekonomika   metody   MeSH
• toxikologie MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• ethylenglykol MeSH
• glycolic acid MeSH Prohlížeč
• glykoláty MeSH

A simple, cost effective, and fast gas chromatography method with flame ionization detection (GC-FID) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicology purposes. This new method employs a relatively less used class of derivatization agents - alkyl chloroformates, allowing the efficient and rapid derivatization of carboxylic acids within seconds while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min. To avoid possible interference from naturally occurring endogenous acids and quantitation errors 3-(4-chlorophenyl) propionic acid was chosen as an internal standard. The significant parameters of the derivatization have been found using chemometric procedures and these parameters were optimized using the face-centered central composite design. The calibration dependence of the method was proved to be quadratic in the range of 50-5000 mg mL(-1), with adequate accuracy (92.4-108.7%) and precision (9.4%). The method was successfully applied to quantify the selected compounds in serum of patients from emergency units.

• Klíčová slova
• Chloroformate, Experimental design, GC, Glycolic acid, Glycols,
• MeSH
• chromatografie plynová metody   MeSH
• ethylenglykol krev   otrava   moč   MeSH
• glykoláty krev   otrava   moč   MeSH
• lidé MeSH
• plamínková ionizace metody   MeSH
• propylenglykol krev   otrava   moč   MeSH
• sérum chemie   MeSH
• studie případů a kontrol MeSH
• toxikologie metody   MeSH
• urgentní zdravotnické služby * MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• ethylenglykol MeSH
• glycolic acid MeSH Prohlížeč
• glykoláty MeSH
• propylenglykol MeSH