A new separation method based on the combination of exclusion and ion exchange chromatography in borate buffer was developed. It allows semi-preparatory and preparatory separation of isobaric N-acylhexosamines (C-2 epimers) and corresponding methyl glycosides (anomers and tautomers). Three types of polyolic gels were tested for these separations. Ion-exchange HPLC was used as a rapid and reliable method for the quantification of the respective analytes. NMR studies of the interactions of N-acetylhexosamines with borate confirmed the importance of a proper stereochemical arrangement of acetamido sugars for their interactions with borate anions.

• MeSH
• boritany chemie   MeSH
• gelová chromatografie metody   MeSH
• hexosaminy chemická syntéza   izolace a purifikace   MeSH
• molekulární sekvence - údaje MeSH
• nukleární magnetická rezonance biomolekulární MeSH
• oligosacharidy chemická syntéza   izolace a purifikace   MeSH
• sacharidové sekvence MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• boritany MeSH
• hexosaminy MeSH
• N-acetylmannosamine MeSH Prohlížeč
• oligosacharidy MeSH