The first indication of the aluminum (Al) toxicity in plants growing in acidic soils is the cessation of root growth, but the detailed mechanism of Al effect is unknown. Here we examined the impact of Al stress on the activity of non-specific phospholipase C (NPC) in the connection with the processes related to the plasma membrane using fluorescently labeled phosphatidylcholine. We observed a rapid and significant decrease of labeled diacylglycerol (DAG), product of NPC activity, in Arabidopsis seedlings treated with AlCl₃. Interestingly, an application of the membrane fluidizer, benzyl alcohol, restored the level of DAG during Al treatment. Our observations suggest that the activity of NPC is affected by Al-induced changes in plasma membrane physical properties.
- Klíčová slova
- Arabidopsis thaliana, BA, benzyl alcohol, BODIPY, BODIPY, 4, 4-difluoro-4-bora-3a, 4a-diaza-s-indacene, BY-2, Bright Yellow 2, DAG, diacylglycerol, HP-TLC, high-performance thin-layer chromatography, MS, Murashige-Skoog, NPC, non-specific phospholipase C, PA, phosphatidic acid, PC, phosphatidylcholine, PC-PLC, phosphatidylcholine-specific phospholipase C, PI-PLC, phosphatidylinositol-specific phospholipase C, PIP2, phosphatidylinositol 4, 5-bisphosphate, PLD, phospholipase D, PM, plasma membrane., aluminum toxicity, benzyl alcohol, diacylglycerol, membrane fluidity, non-specific phospholipase C,
- MeSH
- Arabidopsis účinky léků enzymologie MeSH
- benzylalkohol farmakologie MeSH
- buněčná membrána účinky léků metabolismus MeSH
- diglyceridy metabolismus MeSH
- fosfolipasy typu C metabolismus MeSH
- hliník farmakologie MeSH
- ionty MeSH
- kořeny rostlin účinky léků metabolismus MeSH
- semenáček účinky léků metabolismus MeSH
- sloučeniny boru metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 1,2-diacylglycerol MeSH Prohlížeč
- 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene MeSH Prohlížeč
- benzylalkohol MeSH
- diglyceridy MeSH
- fosfolipasy typu C MeSH
- hliník MeSH
- ionty MeSH
- sloučeniny boru MeSH
