BACKGROUND/AIMS: Branched chain amino acids (BCAAs) are known to exert an insulinotropic effect. Whether this effect is mediated by incretins (glucagon like peptide 1 [GLP-1] or glucose-dependent insulinotropic peptide [GIP]) is not known. The aim of this study was to show whether an equivalent dose of BCAA elicits a greater insulin and incretin response when administered orally than intravenously (IV). METHODS: Eighteen healthy, male subjects participated in 3 tests: IV application of BCAA solution, oral ingestion of BCAA and placebo in an equivalent dose (30.7 ± 1.1 g). Glucose, insulin, C-peptide, glucagon, GLP-1, GIP, valine, leucine and isoleucine concentrations were measured. RESULTS: Rise in serum BCAA was achieved in both BCAA tests, with incremental areas under the curve (iAUC) being 2.1 times greater for IV BCAA compared with those of the oral BCAA test (p < 0.0001). Oral and IV BCAA induced comparable insulin response greater than placebo (240 min insulin iAUC: oral 3,411 ± 577 vs. IV 2,361 ± 384 vs. placebo 961.2 ± 175 pmol/L, p = 0.0006). Oral BCAA induced higher GLP-1 (p < 0.0001) and GIP response (p < 0.0001) compared with the IV or placebo. Glucose levels declined significantly (p < 0.001) in the same pattern during both BCAA tests with no change in the placebo group. CONCLUSIONS: An equivalent dose of BCAA elicited a comparable insulin and greater incretin response when administered orally and not when administered through IV. We conclude that insulinotropic effects of BCAA are partially incretin dependent.

• Klíčová slova
• Branched chain amino acids, Glucagon-like peptide 1, Glucose-dependent insulinotropic peptide, Incretin effect, Insulin response,
• MeSH
• aplikace orální MeSH
• C-peptid krev   MeSH
• dospělí MeSH
• glukagon krev   MeSH
• glukagonu podobný peptid 1 krev   MeSH
• inkretiny krev   MeSH
• intravenózní podání MeSH
• inzulin krev   MeSH
• isoleucin krev   MeSH
• krevní glukóza metabolismus   MeSH
• leucin krev   MeSH
• lidé MeSH
• mladý dospělý MeSH
• valin krev   MeSH
• větvené aminokyseliny aplikace a dávkování   krev   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• žaludeční inhibiční polypeptid krev   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• C-peptid MeSH
• glukagon MeSH
• glukagonu podobný peptid 1 MeSH
• inkretiny MeSH
• inzulin MeSH
• isoleucin MeSH
• krevní glukóza MeSH
• leucin MeSH
• valin MeSH
• větvené aminokyseliny MeSH
• žaludeční inhibiční polypeptid MeSH

The mechanisms behind the changes of body weight after smoking cessation are only partially understood. To this end, we explored the possible effects of smoking cessation on incretin hormones, leptin and selected anthropometric, biochemical and other hormonal parameters. Twenty-two non-obese male adult smokers attending an ambulatory smoking cessation program in Prague, Czech Republic, were examined at the baseline. Thirteen patients (mean age 37.92+/-2.66 years, mean body mass index 25.56+/-0.69 kg/m(2)) successfully quit smoking and were examined three months after smoking cessation; relapsed smokers were not followed up. The patients underwent 2-h liquid meal test with Fresubin and repeated blood sampling for measurements of blood glucose, gastric inhibitory polypeptide (GIP), glucagon-like peptide 1 (GLP-1), amylin, insulin, leptin, peptide-YY (PYY) and pancreatic polypeptide (PP). Three months after smoking cessation, body weight increased (4.35+/-3.32 kg, p<0.001). Leptin levels increased significantly in all repeated samples, while levels of GIP, GLP-1, amylin, insulin, PYY and PP remained unchanged. In conclusions, smoking cessation increased leptin levels probably owing to weight gain while it did not influence incretin levels.

• MeSH
• dospělí MeSH
• inkretiny krev   MeSH
• kouření krev   MeSH
• leptin krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• odvykání kouření * MeSH
• pilotní projekty MeSH
• prospektivní studie MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• inkretiny MeSH
• leptin MeSH

OBJECTIVE: Polycystic ovary syndrome (PCOS) has been linked to a high risk of type 2 diabetes mellitus. Disturbances in the secretion of the incretin hormones glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide 1 (GLP-1) have been observed in states with impaired glucose regulation. This paper considers the secretion of GIP and GLP-1 after oral glucose load in a group of lean, glucose-tolerant PCOS women in comparison with age- and body mass index (BMI)-matched healthy women. DESIGN: Case control. METHODS: PCOS (n=21, 25.8+/-4.1 years, BMI 21.6+/-1.7 kg/m(2)) and control healthy women (CT, n=13, 28.5+/-7.2 years, BMI 20.3+/-2.5 kg/m(2)) underwent oral glucose tolerance test (OGTT) with blood sampling for glucose, insulin, C-peptide, total GIP, and active GLP-1. Insulin sensitivity was determined both at fasting and during the test. STATISTICS: Repeated measures ANOVA. RESULTS: Glucose levels and insulin sensitivity did not differ between PCOS and CT. PCOS had significantly higher levels of C-peptide (P<0.05) and tended to have higher insulin levels. The levels of total GIP were significantly higher in PCOS than in CT (P<0.001). Active GLP-1 levels exhibited a significantly different time-dependent pattern in PCOS (P<0.002 for PCOS versus time interaction). GLP-1 concentrations were similar in PCOS and CT in the early phase of OGTT and then reached significantly lower levels in PCOS than in CT at 180 min (P<0.05). CONCLUSIONS: Increased total GIP and lower late phase active GLP-1 concentrations during OGTT characterize PCOS women with higher C-peptide secretion in comparison with healthy controls, and may be the early markers of a pre-diabetic state.

• MeSH
• C-peptid krev   MeSH
• časové faktory MeSH
• dospělí MeSH
• glukagonu podobný peptid 1 krev   MeSH
• glukózový toleranční test MeSH
• inkretiny krev   MeSH
• inzulin krev   MeSH
• inzulinová rezistence fyziologie   MeSH
• krevní glukóza analýza   MeSH
• lidé MeSH
• studie případů a kontrol MeSH
• syndrom polycystických ovarií krev   MeSH
• žaludeční inhibiční polypeptid krev   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• C-peptid MeSH
• glukagonu podobný peptid 1 MeSH
• inkretiny MeSH
• inzulin MeSH
• krevní glukóza MeSH
• žaludeční inhibiční polypeptid MeSH