We used direct ESI-MS analysis to identify derivatives of cardiolipin molecular species (i.e. O-acyl glycosylated cardiolipins) from the thermophilic bacterium Alicyclobacillus acidoterrestris. We used triple-quadrupole type mass spectrometer for analysis of this complex lipid and enzymatic hydrolysis and 1H and 13C NMR for the identification of these cardiolipin derivatives. These techniques enabled us to identify and quantify the specific molecular species profiles of derivatives of cardiolipin directly from lipid extracts of the bacterium including the identification of the sugar moiety as alpha-D-mannose and all five acyls including their positional isomers.
- MeSH
- Bacteria chemie MeSH
- glykosylace MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací metody MeSH
- hmotnostní spektrometrie metody MeSH
- izotopy kyslíku chemie MeSH
- kardiolipiny analýza izolace a purifikace MeSH
- klinické laboratorní techniky MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- izotopy kyslíku MeSH
- kardiolipiny MeSH
Circular molecules of DNA approximately 3 mum in length were revealed by electron microscopy in deproteinized extracts prepared from purified hydrogenosomal fraction of a protozoan Tritrichomonae foetus. This fraction contained also cardiolipin amounting to approximately 14.4% of its total phospholipids, as detected by thin-layer chromatography and quantitative phosphorus measurement. These characteristics extend a number of biochemical properties of hydrogenosome shared also by mitochondria and by prokaryotic cells.
- MeSH
- kardiolipiny izolace a purifikace MeSH
- kruhová DNA izolace a purifikace MeSH
- organoidy analýza ultrastruktura MeSH
- Tritrichomonas analýza ultrastruktura MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kardiolipiny MeSH
- kruhová DNA MeSH
