Neural rosette formation is a critical morphogenetic process during neural development, whereby neural stem cells are enclosed in rosette niches to equipoise proliferation and differentiation. How neural rosettes form and provide a regulatory micro-environment remains to be elucidated. We employed the human embryonic stem cell-based neural rosette system to investigate the structural development and function of neural rosettes. Our study shows that neural rosette formation consists of five types of morphological change: intercalation, constriction, polarization, elongation and lumen formation. Ca2+ signaling plays a pivotal role in the five steps by regulating the actions of the cytoskeletal complexes, actin, myosin II and tubulin during intercalation, constriction and elongation. These, in turn, control the polarizing elements, ZO-1, PARD3 and β-catenin during polarization and lumen production for neural rosette formation. We further demonstrate that the dismantlement of neural rosettes, mediated by the destruction of cytoskeletal elements, promotes neurogenesis and astrogenesis prematurely, indicating that an intact rosette structure is essential for orderly neural development.
- Klíčová slova
- Adherens junctions, Calcium, Cytoskeleton proteins, Embryonic stem cells, Neural rosette formation, Neural stem cell niche, Neurogenesis,
- MeSH
- aktiny metabolismus MeSH
- apoptóza účinky léků MeSH
- buněčný rodokmen účinky léků MeSH
- cytoskelet účinky léků metabolismus MeSH
- lidé MeSH
- lidské embryonální kmenové buňky cytologie účinky léků metabolismus MeSH
- myosin typu II metabolismus MeSH
- nervové kmenové buňky cytologie účinky léků metabolismus MeSH
- neurogeneze účinky léků MeSH
- neurony cytologie účinky léků metabolismus ultrastruktura MeSH
- polarita buněk účinky léků MeSH
- protein zonula occludens 1 metabolismus MeSH
- tvar buňky * účinky léků MeSH
- tvorba rozet * MeSH
- vápník farmakologie MeSH
- vápníková signalizace * účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- aktiny MeSH
- myosin typu II MeSH
- protein zonula occludens 1 MeSH
- vápník MeSH
