Calcium signaling mediates five types of cell morphological changes to form neural rosettes
Language English Country Great Britain, England Media electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
29361526
DOI
10.1242/jcs.206896
PII: jcs.206896
Knihovny.cz E-resources
- Keywords
- Adherens junctions, Calcium, Cytoskeleton proteins, Embryonic stem cells, Neural rosette formation, Neural stem cell niche, Neurogenesis,
- MeSH
- Actins metabolism MeSH
- Apoptosis drug effects MeSH
- Cell Lineage drug effects MeSH
- Cytoskeleton drug effects metabolism MeSH
- Humans MeSH
- Human Embryonic Stem Cells cytology drug effects metabolism MeSH
- Myosin Type II metabolism MeSH
- Neural Stem Cells cytology drug effects metabolism MeSH
- Neurogenesis drug effects MeSH
- Neurons cytology drug effects metabolism ultrastructure MeSH
- Cell Polarity drug effects MeSH
- Zonula Occludens-1 Protein metabolism MeSH
- Cell Shape * drug effects MeSH
- Rosette Formation * MeSH
- Calcium pharmacology MeSH
- Calcium Signaling * drug effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Actins MeSH
- Myosin Type II MeSH
- Zonula Occludens-1 Protein MeSH
- Calcium MeSH
Neural rosette formation is a critical morphogenetic process during neural development, whereby neural stem cells are enclosed in rosette niches to equipoise proliferation and differentiation. How neural rosettes form and provide a regulatory micro-environment remains to be elucidated. We employed the human embryonic stem cell-based neural rosette system to investigate the structural development and function of neural rosettes. Our study shows that neural rosette formation consists of five types of morphological change: intercalation, constriction, polarization, elongation and lumen formation. Ca2+ signaling plays a pivotal role in the five steps by regulating the actions of the cytoskeletal complexes, actin, myosin II and tubulin during intercalation, constriction and elongation. These, in turn, control the polarizing elements, ZO-1, PARD3 and β-catenin during polarization and lumen production for neural rosette formation. We further demonstrate that the dismantlement of neural rosettes, mediated by the destruction of cytoskeletal elements, promotes neurogenesis and astrogenesis prematurely, indicating that an intact rosette structure is essential for orderly neural development.
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