Autoimmune diseases can be accompanied by presence of various antiphospholipid antibodies (aPL). The laboratory criteria of antiphospholipid syndrome are based on detection of anticardiolipin, lupus anticoagulant or to antiβ2- glycoprotein I but currently a significance of other multiple aPL is being discussed. Because of their vascular and neuroinflammatory effect aPL, if being transplacentally transferred, might inflict damage in developing organism. The aim of our study was to determine the occurrence of eight selected aPL in offspring of mothers with proven autoimmune disease with aPL positivity. The possible influence of aPL presence on clinical, ultrasound and laboratory outcome of children was observed as well. The prospective study included 38 women: 17 women with primary antiphospholipid syndrome and 21 women with other diagnosed autoimmune disease with detected aPL. Also included were 39 children born to the above mentioned mothers between January 2009 and April 2010 in Perinatology Centre in Pilsen, Czech Republic. The control group consisted of 30 mothers without AD and their 30 healthy singletons. Preliminary results of the study showed the presence of aPL in 42.1% neonates of aPL positive mothers with autoimmune disease, six month later aPL were present in only 37.5 % of these children. Observed occurrence of aPL positivity at 6 months of age in originally negative offspring could be attributed to vaccination or food exposure. Psychomotor development of children has proceeded without major deviations. The follow-up study continues and will evaluate both groups of children at two years of age.

• MeSH
• antifosfolipidové protilátky krev   MeSH
• autoimunitní nemoci krev   imunologie   MeSH
• dospělí MeSH
• kojenec MeSH
• komplikace těhotenství krev   imunologie   MeSH
• lidé MeSH
• mladý dospělý MeSH
• novorozenec krev   imunologie   MeSH
• prospektivní studie MeSH
• studie případů a kontrol MeSH
• těhotenství MeSH
• vývoj dítěte MeSH
• zdravotní stav MeSH
• Check Tag
• dospělí MeSH
• kojenec MeSH
• lidé MeSH
• mladý dospělý MeSH
• novorozenec krev   imunologie   MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antifosfolipidové protilátky MeSH

PURPOSE: We followed up lamotrigine transport through the placenta and analyzed maternal and umibilical cord concentrations, its ratio and maternal lamotrigine clearance in monotherapy and in combinations. METHODS: Maternal and umbilical cord concentrations were analyzed during delivery in a cohort of 63 women between 2001 and 2009. The request forms for routine therapeutic drug monitoring were used as the data source. Maternal concentrations were used for the estimation of apparent oral clearance and paired infant and maternal concentrations for estimation of the infant (umibilical cord)/maternal serum concentration ratio. RESULTS: The lamotrigine infant/maternal serum concentration ratio ranged in monotherapy from 0.40 to 1.38 (median 0.91). The ratio in monotherapy showed a possible distribution to two subgroups. Concomitant administration of valproic acid significantly increased both maternal and infant lamotrigine concentrations and significantly decreased lamotrigine clearance by about 65%. Co-medication with carbamazepine increased lamotrigine clearance non-significantly. Highly significant correlations were found between maternal and umbilical cord lamotrigine concentrations, both in monotherapy and in the lamotrigine+valproic acid combination. Infant concentrations of valproic acid were found to be about 30% higher and infant concentrations of carbamazepine were found to be about 20% lower than maternal concentrations. CONCLUSIONS: Our data from the large cohort showed the interindividual variability of umbilical cord/maternal serum concentration ratio in lamotrigine monotherapy caused probably by the different activity of the placental lamotrigine metabolizing enzymes UGT1A4 and 2B7 associated with genetic polymorphism. The potential teratogenic effect of lamotrigine combination with valproic acid could be associated with the higher lamotrigine and valproic acid concentrations in the fetus.

• MeSH
• dospělí MeSH
• epilepsie krev   farmakoterapie   MeSH
• fetální krev metabolismus   MeSH
• kohortové studie MeSH
• lamotrigin MeSH
• lidé MeSH
• maternofetální výměna látek fyziologie   MeSH
• mladiství MeSH
• mladý dospělý MeSH
• novorozenec krev   MeSH
• retrospektivní studie MeSH
• těhotenství krev   MeSH
• triaziny krev   terapeutické užití   MeSH
• vedení porodu * MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• novorozenec krev   MeSH
• těhotenství krev   MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• lamotrigin MeSH
• triaziny MeSH

AIMS: Thymus is the central lymphatic organ in humans with important endocrine function that is involved in maturation of immunocompetent T-lymphocytes. In our study we investigated the relationship among thymus size and the anthropometric dimensions as well as between thymus size and the number of lymphocytes in peripheral blood in full-term newborns. METHODS: The examined group consisted of 212 full-term newborns from the region of Southern Slovakia. We examined birth weight, birth body length, head circumference and chest circumference. Thymus size was estimated by ultrasonography and it was expressed as Thymic Index. The number of lymphocytes in peripheral blood was determined from the number of total leukocytes and from the leukogram. RESULTS: We have found a statistically significant positive correlation among thymus size and the body dimensions of newborns. The highest correlation with thymus size was found for birth weight (r = 0.409; P < 0.001) followed by birth body length (r = 0.368; P < 0.001), head circumference (r = 0.365; P < 0.001) and chest circumference (r = 0.340; P < 0.001). We have proven also a statistically significant positive correlation between the number of lymphocytes in peripheral blood and thymus size (r = 0.208; P = 0.039). CONCLUSION: Our results confirmed the findings of other authors about a close relationship of Thymic Index and basic body parameters in newborns.

• MeSH
• antropometrie * MeSH
• lidé MeSH
• novorozenec krev   MeSH
• počet lymfocytů * MeSH
• thymus anatomie a histologie   MeSH
• velikost orgánu MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• novorozenec krev   MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Slovenská republika MeSH

Carnitine plays an important role in energetic metabolism. The aim of the study was to characterize the carnitine status in term and preterm newborns with respect to gestational age, birth weight, haematocrit and red blood cell count (RBC). The effect of nutrition on carnitine levels in the first week of life was also studied. Total blood pool of free carnitine (FC), acylcarnitines (AC) and total carnitine (TC) were analysed in whole cord blood and postnatally in capillary blood obtained at the day 4-6 in 33 term newborns and at the day 7-10 in 27 preterm newborns using tandem mass spectrometry. Plasma level of carnitine in the cord blood was measured using radioenzymatic method. Cord plasma levels of FC, AC and TC were higher in preterm newborns in comparison with term newborns (p < 0.01), but the total blood pool of FC and TC in whole cord blood was lower in preterm newborns than in term newborns (p < 0.01) and positive correlation was found between FC and gestational age or birth weight (p < 0.05). In addition, positive correlation was found between AC and red blood cell count or haematocrit (p < 0.05). During the first week of life, blood pool of FC and TC in term newborns and AC and TC in preterm newborns decreased regardless of the type of enteral or parenteral nutrition. Our results indicate that preterm newborns are born with limited carnitine store. Interpretation of carnitine analyses in whole blood relies in addition to gestational age and birth weight on the haematocrit, especially in newborns with anaemia or blood hyperviscosity.

• MeSH
• fetální krev chemie   MeSH
• karnitin analogy a deriváty   krev   MeSH
• lidé MeSH
• novorozenec nedonošený krev   MeSH
• novorozenec krev   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• novorozenec krev   MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• acylcarnitine MeSH Prohlížeč
• karnitin MeSH

The study presents a comparison of data concerning lipid metabolism and lipid oxidation (oxidative stress) in children at the time of their birth and 3 months later, as well as of their mothers at the time of delivery, compared to a control group of non-pregnant women of the same age. The data confirm that labour represents an oxidative stress for both mother and child; it is expressed as a significant increase of malondialdehyde concentration in mothers immediately after delivery in comparison with non-pregnant women (p<0.001). Its concentration in newborns was even higher than in their mothers (p<0.005). Concentration of antibodies against oxidized LDL (oxLDLAb) was comparable in mothers and newborns due to their transplacental transport. During the first three months of life these autoantibodies increased almost two-fold. The importance of this unique observation is discussed with respect to possible early atherogenesis.

• MeSH
• autoprotilátky analýza   MeSH
• cholesterol krev   MeSH
• lidé MeSH
• lipoproteiny LDL imunologie   MeSH
• malondialdehyd krev   MeSH
• novorozenec krev   imunologie   MeSH
• oxidační stres MeSH
• superoxiddismutasa krev   MeSH
• volné radikály MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• novorozenec krev   imunologie   MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• autoprotilátky MeSH
• cholesterol MeSH
• lipoproteiny LDL MeSH
• malondialdehyd MeSH
• oxidized low density lipoprotein MeSH Prohlížeč
• superoxiddismutasa MeSH
• volné radikály MeSH

To examine the effects of stressful environmental conditions upon the immune system of the newborn (neonates), we analyzed the neonatal serum immunoglobulin levels in a total of 67 neonates from tribal families living in a rural community of eastern India. These cases were grouped into three categories, based upon the predominance of one of the three factors being analyzed, and the reasonable absence of the other two factors. The three factors as determined by the prevailing environmental conditions, which were the basis for forming the three groups, were: 1. indoor air pollution; 2. hygienic conditions, and 3. the cohabitation of domesticated animals in the same household as the infants's families. Presence of indoor air pollution or unhygienic conditions resulted in the disturbance and depression of the levels of serum immunoglobulins of different classes. There was no discernible correlation found between the levels of immunoglobulins of different classes from the neonates (except IgM) and the cohabitation of domesticated animals in the same households. However the incidence of GIT and RT infections was higher in all the three experimental groups, as compared to the control group. These results suggest that unfavorable environmental conditions can adversely affect the immune system at neonatal stages, and can increase their susceptibility to subsequent acute or chronic events.

• MeSH
• imunoglobuliny krev   MeSH
• kojenec MeSH
• lidé MeSH
• náchylnost k nemoci MeSH
• novorozenec krev   imunologie   MeSH
• pneumonie epidemiologie   MeSH
• počet leukocytů MeSH
• průjem epidemiologie   MeSH
• sanitace * MeSH
• venkovské obyvatelstvo MeSH
• znečištění vzduchu ve vnitřním prostředí * MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH
• novorozenec krev   imunologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• imunoglobuliny MeSH

Eighty-four non-anaemic pregnant women were treated, starting between the 20th-24th week of Pregnancy, with Actiferrin Compositum--1 capsule per day--from the 36th with 2 capsules per day up to childbirth. The group was compared with the results in 57 non-anaemic not treated pregnant women. Haematological parameters were recorded before the onset of treatment, during the first stage of labour and in neonates on the first day after delivery. By means of the non-paired t-test no significant differences were disclosed between the groups; nevertheless the percentage values of haemoglobin, haematocrit, erythrocytes and serum iron were higher in the treated group, as compared with the non-treated one. Also the ferritin values in neonates of the non-treated group were lower, as compared with the treated group. The paired t-test was highly significant in the Actiferrin treated patients as regards haemoglobin, haematocrit and transferrin values. The results provide evidence that it is indicated to administer as a routine measure Actiferrin Compositum to all pregnant women as prevention of pre-partum anaemia of the mother and low ferritin levels in the neonate.

• MeSH
• hematologické komplikace těhotenství prevence a kontrola   MeSH
• hypochromní anemie krev   prevence a kontrola   MeSH
• lidé MeSH
• novorozenec krev   MeSH
• prospektivní studie MeSH
• těhotenství MeSH
• železo terapeutické užití   MeSH
• Check Tag
• lidé MeSH
• novorozenec krev   MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• klinické zkoušky MeSH
• randomizované kontrolované studie MeSH
• Názvy látek
• železo MeSH

The authors investigated C1-inhibitor (C1-INH) plasma levels in blood collected from mothers during delivery: mothers with a normal pregnancy and a group of mothers who displayed during the perinatal period direct or indirect signs of infectious disease. Both groups, as compared with a group of healthy donors, had low C1-INH levels. The neonates were divided into two groups (normal children and children with perinatal risk of infection) with sub-groups of mature full-term neonates (38-41 weeks of gestation) and premature infants (29-36 weeks of gestation). In the group of normal infants the neonates had on average somewhat lower C1-INH levels, as compared with healthy donors, in premature neonates of this group the lowest mean C1-INH level was recorded. In this group the authors observed a correlation between the inhibitor level and the gestation period. The results of C1-INH assessment in the group of neonates with a perinatal risk of infection were different. In premature neonates a higher average C1-INH level was observed than in mature neonates and the relationship between the C1-INH level and the gestation period was a linear negative regression. The postnatal increase of C1-INH levels on the 1st to 5th day was more rapid in premature neonates of both groups.

• MeSH
• gestační stáří MeSH
• infekce krev   MeSH
• infekční komplikace v těhotenství krev   MeSH
• komplement C1 - inaktivátory analýza   MeSH
• lidé MeSH
• novorozenec krev   MeSH
• porodní děj krev   MeSH
• rizikové faktory MeSH
• těhotenství MeSH
• Check Tag
• lidé MeSH
• novorozenec krev   MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• komplement C1 - inaktivátory MeSH

• MeSH
• bilirubin krev   MeSH
• fotometrie metody   MeSH
• lidé MeSH
• novorozenec krev   MeSH
• Check Tag
• lidé MeSH
• novorozenec krev   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• bilirubin MeSH

The authors present values of plasma amino acid concentrations assessed on an automatic analyzer in a group of 12 mature neonates whose early and late post-partum adaptation and subsequent psychosomatic development followed-up to the age of 18 months was normal. The amino acids were assessed at the time of delivery, during the maximal weight loss of the infant after the third day and the last estimation was made before discharge from the maternity hospital when the body weight curves of all infants had a rising trend. None of the infants had neonatal jaundice and all were discharged fully breastfed.

• MeSH
• aminokyseliny krev   MeSH
• kojenec MeSH
• lidé MeSH
• novorozenec krev   MeSH
• referenční hodnoty MeSH
• vývoj dítěte * MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH
• novorozenec krev   MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• aminokyseliny MeSH