Complex metabolic conditions such as type 2 diabetes and obesity result from the interaction of numerous genetic and environmental factors. While the family of Nme proteins has been connected so far mostly to development, proliferation, or ciliary functions, several lines of evidence from human and experimental studies point to the potential involvement of one of its members, NME7 (non-metastatic cells 7, nucleoside diphosphate kinase 7) in carbohydrate and lipid metabolism. As a complete lack of Nme7 is semilethal in rats, we compared morphometric, metabolic, and transcriptomic profiles of standard diet-fed heterozygous Nme7+/- on male rats vs. their wild-type Nme7+/+ controls. Nme7+/- animals showed increased body weight, adiposity, higher insulin levels together with decreased glucose tolerance. Moreover, they displayed pancreatic islet fibrosis and kidney tubular damage. Despite no signs of overt liver steatosis or dyslipidemia, we found significant changes in the hepatic transcriptome of Nme7+/- male rats with a concerted increase of expression of lipogenic enzymes including Scd1, Fads1, Dhcr7 and a decrease of Cyp7b1 and Nme7. Network analyses suggested possible links between Nme7 and the activation of Srebf1 and Srebf2 upstream regulators. These results further support the implication of NME7 in the pathogenesis of glucose intolerance and adiposity.
- Klíčová slova
- animal models, metabolic syndrome, pancreatic fibrosis,
- MeSH
- adipozita genetika MeSH
- diabetes mellitus 2. typu metabolismus MeSH
- dyslipidemie genetika MeSH
- glukosa metabolismus MeSH
- játra metabolismus MeSH
- krysa rodu Rattus MeSH
- lipogeneze genetika MeSH
- metabolismus lipidů fyziologie MeSH
- nukleosiddifosfátkinasa genetika metabolismus MeSH
- obezita metabolismus MeSH
- porucha glukózové tolerance genetika metabolismus MeSH
- potkani Sprague-Dawley MeSH
- transkriptom MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- glukosa MeSH
- NME7 protein, human MeSH Prohlížeč
- nukleosiddifosfátkinasa MeSH
NME7 (non-metastatic cells 7, nucleoside diphosphate kinase 7) is a member of a gene family with a profound effect on health/disease status. NME7 is an established member of the ciliome and contributes to the regulation of the microtubule-organizing center. We aimed to create a rat model to further investigate the phenotypic consequences of Nme7 gene deletion. The CRISPR/Cas9 nuclease system was used for the generation of Sprague Dawley Nme7 knock-out rats targeting the exon 4 of the Nme7 gene. We found the homozygous Nme7 gene deletion to be semi-lethal, as the majority of SDNme7-/- pups died prior to weaning. The most prominent phenotypes in surviving SDNme7-/- animals were hydrocephalus, situs inversus totalis, postnatal growth retardation, and sterility of both sexes. Thinning of the neocortex was histologically evident at 13.5 day of gestation, dilation of all ventricles was detected at birth, and an external sign of hydrocephalus, i.e., doming of the skull, was usually apparent at 2 weeks of age. Heterozygous SDNme7+/- rats developed normally; we did not detect any symptoms of primary ciliary dyskinesia. The transcriptomic profile of liver and lungs corroborated the histological findings, revealing defects in cell function and viability. In summary, the knock-out of the rat Nme7 gene resulted in a range of conditions consistent with the presentation of primary ciliary dyskinesia, supporting the previously implicated role of the centrosomally located Nme7 gene in ciliogenesis and control of ciliary transport.
- Klíčová slova
- Nme7, cilia, hydrocephalus, infertility, knock-out rat,
- MeSH
- cilie metabolismus ultrastruktura MeSH
- fenotyp MeSH
- genetická predispozice k nemoci * MeSH
- genetické asociační studie MeSH
- genotyp MeSH
- genový knockdown MeSH
- imunohistochemie MeSH
- krysa rodu Rattus MeSH
- letální geny * MeSH
- modely nemocí na zvířatech MeSH
- nukleosiddifosfátkinasa nedostatek genetika metabolismus MeSH
- poruchy ciliární motility diagnóza genetika MeSH
- potkani Sprague-Dawley MeSH
- potkani transgenní MeSH
- regulace genové exprese MeSH
- rentgenová mikrotomografie MeSH
- stanovení celkové genové exprese MeSH
- transkriptom MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- nukleosiddifosfátkinasa MeSH
Nucleoside diphosphate kinase 7, non-metastatic cells 7 (NME7) is an acknowledged member of ciliome and is involved in the biogenesis or function of cilia. As obesity and diabetes are common in several ciliopathies, we aimed to analyze changes of gene expression within Nme7 interactome in genetically designed rat models of metabolic syndrome. We assessed the liver transcriptome by Affymetrix microarrays in adult males of 14 PXO recombinant inbred rat strains and their two progenitor strains, SHR-Lx and BXH2. In the strains with the lowest expression of Nme7, we have identified significant enrichment of transcripts belonging to Nme7 interactome. In the subsequent network analysis, we have identified three major upstream regulators - Hnf4a, Ppara and Nr1h4 and liver steatosis (p=0.0001) and liver necrosis/cell death (apoptosis of liver cells, p=0.0003) among the most enriched Tox categories. The mechanistic network reaching the top score showed substantial overlap with Assembly of non-motile cilium and Glucose metabolism disorder gene lists. In summary, we show in a genetic model of metabolic syndrome that rat strains with the lowest expression of Nme7 present gene expression shifts of Nme7 interactome that are perturbing networks relevant for carbohydrate and lipid metabolism as well as ciliogenesis.
- MeSH
- druhová specificita MeSH
- genové regulační sítě genetika MeSH
- krysa rodu Rattus MeSH
- metabolický syndrom genetika metabolismus MeSH
- metabolismus lipidů fyziologie MeSH
- modely nemocí na zvířatech * MeSH
- nukleosiddifosfátkinasa biosyntéza genetika MeSH
- potkani inbrední SHR MeSH
- stanovení celkové genové exprese metody MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- NME7 protein, human MeSH Prohlížeč
- nukleosiddifosfátkinasa MeSH
Nucleoside diphosphate kinases (NDPK) are key enzymes involved in the intracellular nucleotide maintenance in all living organisms, especially in trypanosomatids which are unable to synthesise purines de novo. Four putative NDPK isoforms were identified in the Trypanosoma cruzi Chagas, 1909 genome but only two of them were characterised so far. In this work, we studied a novel isoform from T. cruzi called TcNDPK3. This enzyme presents an atypical N-terminal extension similar to the DM10 domains. In T. cruzi, DM10 sequences targeted other NDPK isoform (TcNDPK2) to the cytoskeleton, but TcNDPK3 was localised in glycosomes despite lacking a typical peroxisomal targeting signal. In addition, TcNDPK3 was found only in the bloodstream trypomastigotes where glycolytic enzymes are very abundant. However, TcNDPK3 mRNA was also detected at lower levels in amastigotes suggesting regulation at protein and mRNA level. Finally, 33 TcNDPK3 gene orthologs were identified in the available kinetoplastid genomes. The characterisation of new glycosomal enzymes provides novel targets for drug development to use in therapies of trypanosomatid associated diseases.
- Klíčová slova
- Chagas disease, energy metabolism, glycosomes, trypanosomatids, trypomastigotes,
- MeSH
- Chagasova nemoc parazitologie MeSH
- energetický metabolismus * MeSH
- fylogeneze MeSH
- izoenzymy MeSH
- mikrotělíska enzymologie MeSH
- nukleosiddifosfátkinasa genetika MeSH
- proteinové domény MeSH
- protozoální proteiny genetika MeSH
- stadia vývoje MeSH
- Trypanosoma cruzi enzymologie genetika fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- izoenzymy MeSH
- nukleosiddifosfátkinasa MeSH
- protozoální proteiny MeSH
