G-quadruplexes have long been perceived as rare and physiologically unimportant nucleic acid structures. However, several studies have revealed their importance in molecular processes, suggesting their possible role in replication and gene expression regulation. Pathways involving G-quadruplexes are intensively studied, especially in the context of human diseases, while their involvement in gene expression regulation in plants remains largely unexplored. Here, we conducted a bioinformatic study and performed a complex circular dichroism measurement to identify a stable G-quadruplex in the gene RPB1, coding for the RNA polymerase II large subunit. We found that this G-quadruplex-forming locus is highly evolutionarily conserved amongst plants sensu lato (Archaeplastida) that share a common ancestor more than one billion years old. Finally, we discussed a new hypothesis regarding G-quadruplexes interacting with UV light in plants to potentially form an additional layer of the regulatory network.
- Klíčová slova
- UV light, circular dichroism, evolution, nucleic acids, plant science,
- MeSH
- Arabidopsis chemie genetika účinky záření MeSH
- cirkulární dichroismus MeSH
- fylogeneze MeSH
- G-kvadruplexy * účinky záření MeSH
- Glaucophyta chemie genetika účinky záření MeSH
- molekulární evoluce MeSH
- regulace genové exprese u rostlin genetika MeSH
- Rhodophyta chemie genetika účinky záření MeSH
- RNA-polymerasa II chemie genetika MeSH
- rostlinné proteiny chemie genetika účinky záření MeSH
- rostliny chemie genetika účinky záření MeSH
- sekvence aminokyselin MeSH
- sekvenční seřazení MeSH
- ultrafialové záření MeSH
- výpočetní biologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- RNA-polymerasa II MeSH
- rostlinné proteiny MeSH
