A nonpathogenic bacterium of external environment possessing remarkable immunomodulatory activity, Bacillus firmus (BF) inactivated with formaldehyde, was given intragastrically to two genetically different mouse strains BALB/c (H-2d) and B10.BR/SnPh (B10.BR, H-2k) reared in conventional (CV) and B10.BR strain also in germ-free (GF) conditions. Repeated intragastric administration of BF (500 micrograms every other day over two weeks, starting at the age of 3 months) significantly enhanced intestinal IgA levels in CV BALB/c mice but did not affect intestinal IgA in CV B10.BR mice. In GF B10.BR mice, IgG levels in sera and intestinal washings increased after BF administration compared to CV B10.BR mice. In CV BALB/c mice, specific activity of enterocyte brush-border enzymes (lactase, gamma-glutamyltransferase, alkaline phosphatase) decreased after BF treatment; sucrase (sucrose alpha-glucosidase) activity was not affected. On the other hand, in B10.BR mice, specific activity of gamma-glutamyltransferase and dipeptidyl peptidase IV were higher after administration of BF in both CV and GF groups relative to untreated controls. The activities of lactase and glucoamylase (glucan 1,4-alpha-glucosidase) were significantly stimulated only in the group of GF B10.BR mice treated with formolized BF. The stimulation of immunoglobulin production after BF treatment was accompanied by changes in the levels of enterocyte brush-border enzymes; this responsiveness to BF treatment was genetically regulated.

• MeSH
• alkalická fosfatasa metabolismus   MeSH
• Bacillus imunologie   MeSH
• beta-galaktosidasa metabolismus   MeSH
• dipeptidylpeptidasa 4 metabolismus   MeSH
• enterocyty enzymologie   mikrobiologie   MeSH
• genetická predispozice k nemoci MeSH
• glukoamylasa metabolismus   MeSH
• gnotobiologické modely MeSH
• imunoglobulin A biosyntéza   krev   MeSH
• imunohistochemie MeSH
• laktasa MeSH
• mikroklky enzymologie   MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• sacharasa metabolismus   MeSH
• střeva enzymologie   imunologie   mikrobiologie   MeSH
• střevní sliznice enzymologie   imunologie   mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• alkalická fosfatasa MeSH
• beta-galaktosidasa MeSH
• dipeptidylpeptidasa 4 MeSH
• glukoamylasa MeSH
• imunoglobulin A MeSH
• laktasa MeSH
• sacharasa MeSH