The pig amnion was in vivo intraamniotically infected with E. coli for 10 h at 80-85 d of gestation either with the nonpathogenic O86 strain or enteropathogenic O55 strain. TNF-alpha, IL-10, IL-1beta and IFN-gamma were determined in amniotic fluids by ELISA, the expression of cytokines and some other inflammatory markers was determined by immunohistochemistry. Intraamniotic infection induced high levels of TNF-alpha in amniotic fluids which correlated with bacterial virulence whereas IL-10 was induced only by O86. The IL-1beta level did not increase significantly and was expressed in all infected membranes. IFN-gamma was negligible or absent. TNF-alpha, IL-12p40, calprotectin, HSP65 and gp91phox were found by immunohistochemistry only in amnion membranes infected with the enteropathogenic strain 055.

• MeSH
• amnion imunologie   mikrobiologie   MeSH
• biologické markery metabolismus   MeSH
• cytokiny metabolismus   MeSH
• Escherichia coli imunologie   patogenita   MeSH
• infekce vyvolané Escherichia coli imunologie   mikrobiologie   veterinární   MeSH
• infekční komplikace v těhotenství imunologie   mikrobiologie   veterinární   MeSH
• interferon gama metabolismus   MeSH
• interleukin-1 metabolismus   MeSH
• interleukin-10 metabolismus   MeSH
• nemoci prasat imunologie   mikrobiologie   MeSH
• prasata MeSH
• těhotenství MeSH
• TNF-alfa metabolismus   MeSH
• zánět imunologie   MeSH
• zvířata MeSH
• Check Tag
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biologické markery MeSH
• cytokiny MeSH
• interferon gama MeSH
• interleukin-1 MeSH
• interleukin-10 MeSH
• TNF-alfa MeSH