The widely used fluorescent probe 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) serves as a pH-sensitive indicator in classical microscopy. Characteristics of BCECF were studied and a way of employing the probe in a confocal laser scanning microscope equipped with an argon laser at 488 nm was developed, based on the fact that the emission fluorescence spectra are pH-dependent with spectral maximum shift from 518 to 529 nm. Optical filters for the dual-emission ratio method were set to 506 and 529 nm. pH values measured inside a single cell of Saccharomyces cerevisiae were similar to those obtained with other pH estimation methods.

• MeSH
• Argon MeSH
• Fluoresceins * MeSH
• Fluorescent Dyes * MeSH
• Spectrometry, Fluorescence MeSH
• Intracellular Fluid metabolism   MeSH
• Hydrogen-Ion Concentration MeSH
• Microscopy, Confocal methods   MeSH
• Lasers MeSH
• Flow Cytometry methods   MeSH
• Saccharomyces cerevisiae metabolism   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Comparative Study MeSH
• Names of Substances
• 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein MeSH Browser
• Argon MeSH
• Fluoresceins * MeSH
• Fluorescent Dyes * MeSH