Nejvíce citovaný článek - PubMed ID 10756518
Three strains of Escherichia fergusonii (EF873, EF1496, EF939) of 50 strains tested produced the hydroxamate siderophore aerobactin. Screening of a cosmid library of the strain EF873 chromosomal DNA (in aerobactin nonproducing Escherichia coli VCS257) for aerobactin production identified iucABCD and iutA gene orthologues. The predicted IucABCD and IutA proteins showed 59-65% identity to the corresponding proteins of Shigella flexneri and E. coli. Aerobactin molecules synthesized by E. fergusonii and E. coli strains stimulated growth of aerobactin indicator strains harboring either E. coli or E. fergusonii iutA genes. In the 12 kb upstream and 17 kb downstream regions of the iuc and iut genes, 20 additional ORFs were identified. Their gene products showed homology to proteins from E. coli, S. flexneri, Klebsiella aerogenes, Pseudomonas aeruginosa and Vibrio cholerae. Probes recognizing DNA sequences from a region of more than 25 kb, which included the iucABCD and iutA genes, hybridized with chromosomal DNA of two aerobactin-producing strains (EF873 and EF939), but not with other nonproducing E. fergusonii strains tested. These data, together with the genetic organization of this region, suggest that E. fergusonii iucABCD iutA genes are a portion of a larger segment of DNA similar to pathogenicity islands of other bacteria.
- MeSH
- bakteriální chromozomy genetika MeSH
- Escherichia genetika metabolismus patogenita MeSH
- genomová knihovna MeSH
- inzerční mutageneze MeSH
- kosmidy MeSH
- kyseliny hydroxamové metabolismus MeSH
- mapování chromozomů * MeSH
- molekulární sekvence - údaje MeSH
- proteiny vnější bakteriální membrány genetika metabolismus MeSH
- proteiny z Escherichia coli genetika metabolismus MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA MeSH
- Southernův blotting MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Názvy látek
- aerobactin receptor MeSH Prohlížeč
- aerobactin MeSH Prohlížeč
- kyseliny hydroxamové MeSH
- proteiny vnější bakteriální membrány MeSH
- proteiny z Escherichia coli MeSH
