Eleven type strains and 24 Lactobacillus isolates, preliminarily classified to the species due to phenotypic features, were investigated. Standard methods of identification with species-specific PCRs and typing with PFGE (with ApaI, NotI and SmaI restriction enzymes) allowed us to distinguish 16 unique strains belonging to 5 species (L. acidophilus, L. delbrueckii ssp. bulgaricus, L. plantarum, L. rhamnosus, L. salivarius). Alternative approach with 16S-23S rDNA ARDRA identification (with merely two restrictases, BsuRI and TaqI) and PCR-based typing (RAPD with two random- and rep-PCR with (GTG)(5) primers) showed to be more discriminative, i.e. 21 unique strains were classified in the same species as above. As a result, 7 out of 24 phenotypically species-assigned isolates were reclassified. The alternative procedure of rapid identification and typing of Lactobacillus isolates appeared to be equally effective and shortened from 1 week to 2-3 d (in comparison to the standard methods).

• MeSH
• časové faktory MeSH
• DNA bakterií analýza   genetika   MeSH
• DNA primery MeSH
• druhová specificita MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• fenotyp MeSH
• Lactobacillus klasifikace   genetika   izolace a purifikace   MeSH
• polymerázová řetězová reakce metody   MeSH
• restrikční mapování metody   MeSH
• RNA ribozomální 16S genetika   MeSH
• RNA ribozomální 23S genetika   MeSH
• technika náhodné amplifikace polymorfní DNA metody   MeSH
• techniky typizace bakterií metody   MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• Názvy látek
• DNA bakterií MeSH
• DNA primery MeSH
• RNA ribozomální 16S MeSH
• RNA ribozomální 23S MeSH