Rapid molecular identification and characteristics of Lactobacillus strains
Language English Country United States Media print-electronic
Document type Evaluation Study, Journal Article
- MeSH
- Time Factors MeSH
- DNA, Bacterial analysis genetics MeSH
- DNA Primers MeSH
- Species Specificity MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Phenotype MeSH
- Lactobacillus classification genetics isolation & purification MeSH
- Polymerase Chain Reaction methods MeSH
- Restriction Mapping methods MeSH
- RNA, Ribosomal, 16S genetics MeSH
- RNA, Ribosomal, 23S genetics MeSH
- Random Amplified Polymorphic DNA Technique methods MeSH
- Bacterial Typing Techniques methods MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Names of Substances
- DNA, Bacterial MeSH
- DNA Primers MeSH
- RNA, Ribosomal, 16S MeSH
- RNA, Ribosomal, 23S MeSH
Eleven type strains and 24 Lactobacillus isolates, preliminarily classified to the species due to phenotypic features, were investigated. Standard methods of identification with species-specific PCRs and typing with PFGE (with ApaI, NotI and SmaI restriction enzymes) allowed us to distinguish 16 unique strains belonging to 5 species (L. acidophilus, L. delbrueckii ssp. bulgaricus, L. plantarum, L. rhamnosus, L. salivarius). Alternative approach with 16S-23S rDNA ARDRA identification (with merely two restrictases, BsuRI and TaqI) and PCR-based typing (RAPD with two random- and rep-PCR with (GTG)(5) primers) showed to be more discriminative, i.e. 21 unique strains were classified in the same species as above. As a result, 7 out of 24 phenotypically species-assigned isolates were reclassified. The alternative procedure of rapid identification and typing of Lactobacillus isolates appeared to be equally effective and shortened from 1 week to 2-3 d (in comparison to the standard methods).
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