A manipulative experiment with two different water regimes was established to identify the variability of physiological responses to environmental changes in 5-year-old Norway spruce provenances in the Western Carpathians. While variations in the growth responses were detected only between treatments, photosynthetic and biochemical parameters were also differently influenced among provenances. Following drought treatment, an obvious shrinkage of tree stems was observed. In most provenances, drought had a negative effect on leaf gas-exchange parameters and kinetics of chlorophyll a fluorescence. Secondary metabolism was not affected so much with notable differences in concentration of sabinene, o-cimene, and (-)-alpha-terpineol monoterpenes. The most suitable indicators of drought stress were abscisic acid and fluorescence parameters. Seedlings from the highest altitude (1,500 m a.s.l.) responded better to stress conditions than the other populations. Such provenance trials may be a valuable tool in assessing the adaptive potential of spruce populations under changing climate.

• Klíčová slova
• Norway spruce, abscisic acid, drought, monoterpenes, photosynthesis, provenance,
• Publikační typ
• časopisecké články MeSH

Phloem osmolality and its components are involved in basic cell metabolism, cell growth, and in various physiological processes including the ability of living cells to withstand drought and frost. Osmolality and sugar composition responses to environmental stresses have been extensively studied for leaves, but less for the secondary phloem of plant stems and branches. Leaf osmotic concentration and the share of pinitol and raffinose among soluble sugars increase with increasing drought or cold stress, and osmotic concentration is adjusted with osmoregulation. We hypothesize that similar responses occur in the secondary phloem of branches. We collected living bark samples from branches of adult Pinus sylvestris, Picea abies, Betula pendula and Populus tremula trees across Europe, from boreal Northern Finland to Mediterranean Portugal. In all studied species, the observed variation in phloem osmolality was mainly driven by variation in phloem water content, while tissue solute content was rather constant across regions. Osmoregulation, in which osmolality is controlled by variable tissue solute content, was stronger for Betula and Populus in comparison to the evergreen conifers. Osmolality was lowest in mid-latitude region, and from there increased by 37% toward northern Europe and 38% toward southern Europe due to low phloem water content in these regions. The ratio of raffinose to all soluble sugars was negligible at mid-latitudes and increased toward north and south, reflecting its role in cold and drought tolerance. For pinitol, another sugar known for contributing to stress tolerance, no such latitudinal pattern was observed. The proportion of sucrose was remarkably low and that of hexoses (i.e., glucose and fructose) high at mid-latitudes. The ratio of starch to all non-structural carbohydrates increased toward the northern latitudes in agreement with the build-up of osmotically inactive C reservoir that can be converted into soluble sugars during winter acclimation in these cold regions. Present results for the secondary phloem of trees suggest that adjustment with tissue water content plays an important role in osmolality dynamics. Furthermore, trees acclimated to dry and cold climate showed high phloem osmolality and raffinose proportion.

• Klíčová slova
• hexose, osmotic concentration, phloem water content, pinitol, raffinose, starch, sucrose,
• Publikační typ
• časopisecké články MeSH