Auxin phytohormones control most aspects of plant development through a complex and interconnected signaling network. In the presence of auxin, AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors are targeted for degradation by the SKP1-CULLIN1-F-BOX (SCF) ubiquitin-protein ligases containing TRANSPORT INHIBITOR RESISTANT 1/AUXIN SIGNALING F-BOX (TIR1/AFB). CULLIN1-neddylation is required for SCFTIR1/AFB functionality, as exemplified by mutants deficient in the NEDD8-activating enzyme subunit AUXIN-RESISTANT 1 (AXR1). Here, we report a chemical biology screen that identifies small molecules requiring AXR1 to modulate plant development. We selected four molecules of interest, RubNeddin 1 to 4 (RN1 to -4), among which RN3 and RN4 trigger selective auxin responses at transcriptional, biochemical, and morphological levels. This selective activity is explained by their ability to consistently promote the interaction between TIR1 and a specific subset of AUX/IAA proteins, stimulating the degradation of particular AUX/IAA combinations. Finally, we performed a genetic screen using RN4, the RN with the greatest potential for dissecting auxin perception, which revealed that the chromatin remodeling ATPase BRAHMA is implicated in auxin-mediated apical hook development. These results demonstrate the power of selective auxin agonists to dissect auxin perception for plant developmental functions, as well as offering opportunities to discover new molecular players involved in auxin responses.

• Klíčová slova
• auxin, chemical biology, hormone perception, prohormone, selective agonist,
• MeSH
• Arabidopsis genetika   metabolismus   MeSH
• F-box proteiny metabolismus   MeSH
• genetická transkripce účinky léků   MeSH
• geneticky modifikované rostliny genetika   MeSH
• kyseliny indoloctové metabolismus   MeSH
• protein NEDD8 genetika   MeSH
• proteinligasy komplexu SCF metabolismus   MeSH
• proteiny huseníčku genetika   metabolismus   MeSH
• proteolýza * MeSH
• receptory buněčného povrchu metabolismus   MeSH
• regulace genové exprese u rostlin MeSH
• regulátory růstu rostlin genetika   metabolismus   MeSH
• semenáček metabolismus   MeSH
• signální transdukce MeSH
• transkripční faktory metabolismus   MeSH
• vývoj rostlin genetika   fyziologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH
• Research Support, U.S. Gov't, Non-P.H.S. MeSH
• Názvy látek
• F-box proteiny MeSH
• indoleacetic acid MeSH Prohlížeč
• kyseliny indoloctové MeSH
• protein NEDD8 MeSH
• proteinligasy komplexu SCF MeSH
• proteiny huseníčku MeSH
• receptory buněčného povrchu MeSH
• regulátory růstu rostlin MeSH
• transkripční faktory MeSH