The somatic cuticle of adult female Philometra obturans (Prenant, 1886) (Nematoda: Dracunculoidea) consists of five zones and an overlying prominent fuzzy epicuticle, closely adherent to the gill arterial wall of the host. The cuticle is fenestrated and infiltrated with electron-dense substances. It protrudes into the hypodermis in the form of numerous protuberances. The plasma membrane of the hypodermis forms prominent infoldings within the subcuticular region. Numerous endosomes bud off from the cuticle into the hypodermis. Glycogen deposits are present within the hypodermis and muscle cells. The muscle cells are well developed, polymyarian in number (up to 30 in each quadrant) and coelomyarian in shape. The body wall of adult female P. obturans reveals several ultrastructural features which are common to the members of Filarioidea.

• MeSH
• arterie parazitologie   MeSH
• Dracunculoidea metabolismus   ultrastruktura   MeSH
• Esocidae parazitologie   MeSH
• infekce hlísticemi řádu Spirurida parazitologie   veterinární   MeSH
• mikrotomie metody   MeSH
• nemoci ryb parazitologie   MeSH
• transmisní elektronová mikroskopie MeSH
• žábry krevní zásobení   parazitologie   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The intestinal epithelium of third-stage larvae and adults of Cystidicoloides ephemeridarum from haemocoel of mayflies and stomach of brown trout was studied by electron microscopy and cytochemistry. In section, the intestine of both stages is composed of a single layer of about ten undifferentiated intestinal cells in a ring. A labyrinth of deep invaginations is present in the basal region of each cell. The apical surface is modified into well developed, regularly arranged microvilli. These, together with numerous organelles engaged in metabolism and a well defined gut lumen filled with unidentifiable material suggest that the intestine may function in digestion and absorption during both stages. The adults seem to feed upon the semifluid content of the stomach of brown trout. Fortuitous oral infection with undetermined bacteria in vitro led to degenerative changes in the intestinal tissue and probably caused death of the infected specimens. Up to 75% of the cell volume in the L(3) is occupied by glycogen deposits. In the adults, a minor portion of glycogen, together with lipid droplets, has been observed. The adults are considered to rely more on aerobic metabolism, whereas anaerobic metabolism (glycolysis) may prevail in L(3).

• MeSH
• infekce hlísticemi řádu Spirurida parazitologie   veterinární   MeSH
• larva ultrastruktura   MeSH
• nemoci ryb parazitologie   MeSH
• pstruh parazitologie   MeSH
• Spirurida růst a vývoj   ultrastruktura   MeSH
• střeva ultrastruktura   MeSH
• světluškovití parazitologie   MeSH
• transmisní elektronová mikroskopie MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH

Scanning and transmission electron microscopic examinations of tissue-dwelling third-stage larvae of the nematode Cystidicoloides ephemeridarum from the intermediate host (Ephemera danica) were carried out with respect to the morphological changes in the body wall associated with the transfer from the intermediate to the definitive host. The ultrastructure of the cephalic end and zonation of the somatic cuticle of infective larvae basically correspond with those of adults. The somatic cuticle is composed of a fuzzy epicuticle, an outer and inner cortical zone, a median zone and a basal zone consisting of three subzones. Globular bodies are absent from the median zone of the infective larvae. The lateral hypodermal cords of the infective larvae are cellular, consisting of a median cell enclosed by two sublateral cells. The excretory canal is present within each of the lateral cord in both the infective and adult stages. Walls of the excretory canals contain Golgi-derived vesicles which communicate with the lumen of the canal. Large deposits of glycogen, suggesting anaerobic respiration, are present in the hypodermal cords and noncontractile parts of the muscle cells of the third-stage larvae within the intermediate host.

• MeSH
• larva patogenita   ultrastruktura   MeSH
• mikroskopie elektronová rastrovací MeSH
• Spirurida růst a vývoj   patogenita   ultrastruktura   MeSH
• světluškovití parazitologie   MeSH
• transmisní elektronová mikroskopie MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Light, scanning and transmission electron microscope examinations of adult Cystidicoloides ephemeridarum nematodes, parasitic in the stomach of brown trout (Salmo trutta fario L.), revealed that the body wall is composed of a cuticle, a hypodermis and longitudinally oriented somatic musculature. The body cuticle is composed of a trilaminated epicuticle, an outer and inner cortical zone, a median zone with globular bodies and a basal zone consisting of three subzones. Massive cuticle in the three zones and an osmiophilic lining is present in the buccal capsule. Armament is absent both from the buccal capsule and the head end. The surface of the body is transversely striated with cortical annuli. The only specialized attachment devices of C. ephemeridarum are the flexible overlapping margins of the annuli which are elongated in the first third of the body. Together with the sucking pressure of the oesophagus, they seem to play a role in the penetration and mechanical damage of the host's tissue. The hypodermis appears to be syncytial. Up to 300 worms were present in the bolus of food consumed by the host, or attached by their head ends in the lamina epithelialis, which resulted in localized disruptions of the mucosal epithelium with no inflammation. Infection with C. ephemeridarum seems to be a temporary, season-dependent event of mild pathogenicity.

• MeSH
• elektronová mikroskopie MeSH
• infekce hlísticemi řádu Spirurida parazitologie   patologie   veterinární   MeSH
• mikroskopie elektronová rastrovací MeSH
• nemoci ryb parazitologie   patologie   MeSH
• pstruh parazitologie   MeSH
• Spirurida patogenita   ultrastruktura   MeSH
• žaludek parazitologie   patologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH