Most cited article - PubMed ID 11403849
The Zygosaccharomyces rouxii strain CBS732 contains only one copy of the HOG1 and the SOD2 genes
The osmotolerant and cell wall properties of the two most studied wild-type Zygosaccharomyces rouxii strains (CBS 732 and ATCC 42981) were examined. Differences in their (1) tolerance to high salt content in the medium, (2) resistance to the lysing enzymes Lyticase and Zymolyase, (3) cell-wall polymer content and (4) cell wall micromorphology suggested that the less osmotolerant CBS 732 strain possesses a more rigid cell wall than the more osmotolerant ATCC 42981, whose cell wall seems to be more flexible and elastic.
- MeSH
- Antifungal Agents pharmacology MeSH
- Cell Wall physiology ultrastructure MeSH
- Glucan Endo-1,3-beta-D-Glucosidase pharmacology MeSH
- Adaptation, Physiological MeSH
- Saline Solution, Hypertonic metabolism MeSH
- Multienzyme Complexes pharmacology MeSH
- Osmolar Concentration MeSH
- Peptide Hydrolases pharmacology MeSH
- Saccharomyces cerevisiae physiology MeSH
- Zygosaccharomyces cytology physiology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antifungal Agents MeSH
- Glucan Endo-1,3-beta-D-Glucosidase MeSH
- Saline Solution, Hypertonic MeSH
- lyticase MeSH Browser
- Multienzyme Complexes MeSH
- Peptide Hydrolases MeSH
Yarrowia lipolytica plasma-membrane Na+/H+ antiporter, encoded by the YlNHA2 gene, is a very efficient exporter of surplus sodium from the cytosol. Its heterologous expression in Saccharomyces cerevisiae wild-type laboratory strains increased their sodium tolerance more efficiently than the expression of ZrSod2-22 antiporter from the osmotolerant yeast Zygosaccharomvces rouxii.
- MeSH
- Antifungal Agents pharmacology MeSH
- Gene Expression MeSH
- Fungal Proteins genetics metabolism MeSH
- Cloning, Molecular MeSH
- Sodium-Hydrogen Exchangers genetics metabolism MeSH
- Recombinant Proteins genetics metabolism MeSH
- Saccharomyces cerevisiae drug effects genetics growth & development metabolism MeSH
- Salts pharmacology MeSH
- Yarrowia enzymology genetics MeSH
- Zygosaccharomyces enzymology genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antifungal Agents MeSH
- Fungal Proteins MeSH
- Sodium-Hydrogen Exchangers MeSH
- Recombinant Proteins MeSH
- Salts MeSH
The Saccharomyces cerevisiae genome contains three genes encoding alkali metal cation/H+ antiporters (Nha1p, Nhx1p, Kha1p) that differ in cell localization, substrate specificity and physiological function. Systematic genome sequencing of other yeast species revealed highly conserved homologous ORFs in all of them. We compared the yeast sequences both at DNA and protein levels. The subfamily of yeast endosomal/prevacuolar Nhx1 antiporters is closely related to mammalian plasma membrane NHE proteins and to both plasma membrane and vacuolar plant antiporters. The high sequence conservation within this subfamily of yeast antiporters suggests that Nhx1p is of great importance in cell physiology. Yeast Kha1 proteins probably belong to the same subfamily as bacterial antiporters, whereas Nhal proteins form a distinct subfamily.
- MeSH
- DNA, Fungal analysis MeSH
- Potassium-Hydrogen Antiporters chemistry classification genetics MeSH
- Phylogeny MeSH
- Membrane Proteins chemistry classification genetics MeSH
- Molecular Sequence Data MeSH
- Sodium-Hydrogen Exchangers chemistry classification genetics MeSH
- Cation Transport Proteins chemistry classification genetics MeSH
- Saccharomyces cerevisiae Proteins chemistry classification genetics MeSH
- Amino Acid Sequence MeSH
- Sequence Analysis, DNA MeSH
- Sequence Homology, Amino Acid MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Fungal MeSH
- Potassium-Hydrogen Antiporters MeSH
- KHA1 protein, S cerevisiae MeSH Browser
- Membrane Proteins MeSH
- Sodium-Hydrogen Exchangers MeSH
- NHA1 protein, S cerevisiae MeSH Browser
- NHX1 protein, S cerevisiae MeSH Browser
- Cation Transport Proteins MeSH
- Saccharomyces cerevisiae Proteins MeSH