The alkaloids N-methylhydrasteine hydroxylactam and 1-methoxyberberine chloride were isolated from Corydalis longipes. Both alkaloids showed high efficacy individually (in concentration of 50-150 ppm) and also in a 1:1 mixture against spore germination of some fungi, viz. Alternaria alternata, A. brassicae, Curvularia maculans, Curvularia sp., Colletotrichum gloeosporioides, Colletotrichum sp., Helminthosporium speciferum, H. pennisetti, Helminthosporium sp., and Ustilago cynodontis. The antifungal effect of single compounds was dose-dependent. If the mutual ratio of the two components in the mixture was changed from 1:1 to a major content of any of the two compounds, the inhibitory effect on spore germination decreased.

• MeSH
• Alkaloids chemistry   isolation & purification   pharmacology   MeSH
• Antifungal Agents chemistry   isolation & purification   pharmacology   MeSH
• Corydalis chemistry   MeSH
• Fungi drug effects   growth & development   MeSH
• Plant Preparations chemistry   MeSH
• Spores, Fungal drug effects   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Alkaloids MeSH
• Antifungal Agents MeSH
• Plant Preparations MeSH

(+/-)-alpha-Hydrastine and (+/-)-beta-hydrastine were isolated from Corydalis longipes; both exhibited considerable efficacy against spore germination of some saprophytic and phytopathogenic fungi. While (+/-)-alpha-hydrastine was effective against most of the fungi, Helminthosporium echinoclova was least affected even at the highest dose (150 ppm). (+/-)-beta-Hydrastine was equally effective against several fungi. Mixture of both compounds was more effective than each one individually. Helminthosporium species were again the most resistant toward the mixture. The effect of both alkaloids independently on germination and development of E. pisi conidia on excised pea leaves was also shown. After pre-inoculation with (+/-)-alpha-hydrastine, the effect was more pronounced than the addition post-inoculation; maximum inhibition occurred at 200 ppm. (+/-)-beta-Hydrastine also reduced germination of conidia but was less effective than (+/-)-alpha-hydrastine. The number of primary and secondary branches of conidia and number of appressoria were not affected significantly by either compound.

• MeSH
• Alkaloids pharmacology   MeSH
• Antifungal Agents pharmacology   MeSH
• Benzylisoquinolines MeSH
• Pest Control, Biological methods   MeSH
• Corydalis microbiology   MeSH
• Germination drug effects   MeSH
• Plant Leaves microbiology   MeSH
• Mitosporic Fungi drug effects   growth & development   MeSH
• Plant Diseases microbiology   MeSH
• Spores, Fungal drug effects   growth & development   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Alkaloids MeSH
• Antifungal Agents MeSH
• Benzylisoquinolines MeSH
• hydrastine MeSH Browser

The antimicrobial activity of the protoberberine alkaloid, berberine, isolated from Mahonia aquifolium, was evaluated against 17 microorganisms including two Gram-negative bacteria--Pseudomonas aeruginosa and Escherichia coli (both resistant and sensitive), two Gram-positive bacteria--Bacillus subtilis and Staphylococcus aureus, Zoogloea ramigera, six filamentous fungi--Penicilium chrysogenum, Aspergillus niger, Aureobasidium pullulans (black and white strain), Trichoderma viride (original green strain and brown mutant), Fusarium nivale, Mycrosporum gypseum and two yeasts--Candida albicans and Saccharomyces cerevisiae. The IC50, minimum inhibitory concentration (MIC), minimum microbicidal concentration (MMC) and minimum microbistatic concentration (MMS) varied considerably depending on the microorganism tested, the sensitivity decreasing as follows: S. aureus > P. aeruginosa S (sensitive) > E. coli S > P. aeruginosa R (resistant) > E. coli R > B. subtilis > Z. ramigera > C. albicans > S. cerevisiae > A. pullulans B (black) > A. pullulans W (white) > T. viride Br (brown) > M. gypseum > A. niger > F. nivale > P. chrysogenum > T. viride G (green).

• MeSH
• Anti-Bacterial Agents MeSH
• Drug Resistance, Microbial MeSH
• Antifungal Agents isolation & purification   pharmacology   MeSH
• Anti-Infective Agents isolation & purification   pharmacology   MeSH
• Berberine isolation & purification   pharmacology   MeSH
• Gram-Negative Bacteria drug effects   MeSH
• Gram-Positive Bacteria drug effects   MeSH
• Fungi drug effects   MeSH
• Yeasts drug effects   MeSH
• Mahonia chemistry   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH
• Antifungal Agents MeSH
• Anti-Infective Agents MeSH
• Berberine MeSH

Inhibition activity of the alkaloid (-)-corypalmine on spore germination of plant pathogenic and saprophytic fungi (Alternaria solani, A brassicicola, A. brassicae, A. melongenae, Curvularia pallescens, C. lunata, C. maculans, Curvularisa sp., Colletotrichum sp., Helminthosporium speciferum, H. frumentacei, H. pennisetti, Heterosporium sp., Penicillum sp., Ustilago cynodontis) was determined. Spore germination of all the tested fungi was inhibited, Heterosporium sp. and Ustilago cynodontis being the most sensitive (complete inhibition of spore germination was observed at the very low concentration of 200 ppm). Curvularia palliscens, C. maculans and Curvularia sp. were less sensitive; complete inhibition of spore germination occurred at 400 ppm.

• MeSH
• Alkaloids chemistry   isolation & purification   pharmacology   MeSH
• Antifungal Agents isolation & purification   pharmacology   MeSH
• Species Specificity MeSH
• Heterocyclic Compounds, 4 or More Rings pharmacology   MeSH
• Fungi drug effects   physiology   MeSH
• Plant Extracts chemistry   pharmacology   MeSH
• Spores, Fungal drug effects   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Alkaloids MeSH
• Antifungal Agents MeSH
• corypalmine MeSH Browser
• Heterocyclic Compounds, 4 or More Rings MeSH
• Plant Extracts MeSH