Three hydroxyl-radical producing biomimetic systems, composed of CuII, hydrogen peroxide and pyridine, glucaric or succinic acid, were able to perform decolorization of olive mill wastewaters (OMW) >85 % within 3 d combined with a significant removal of total phenols (>75 %). The systems consisting of 50 mmol/L succinic acid, 5-10 mmol/L CuSO4 and 100 mmol/L H2O2 were the most effective at OMW treatment, and led to the reduction of phenol contents to <1 % along with high decolorization (>88 %) and acceptable values of chemical oxygen demand.

• MeSH
• Bacteria metabolism   MeSH
• Biodegradation, Environmental MeSH
• Pigments, Biological chemistry   MeSH
• Biomimetics methods   MeSH
• Phenols chemistry   MeSH
• Hydroxyl Radical chemistry   MeSH
• Copper MeSH
• Waste Disposal, Fluid methods   MeSH
• Plant Oils * MeSH
• Olive Oil MeSH
• Oxidation-Reduction MeSH
• Hydrogen Peroxide chemistry   MeSH
• Industrial Waste MeSH
• Free Radicals pharmacology   MeSH
• Iron chemistry   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Pigments, Biological MeSH
• Phenols MeSH
• Fenton's reagent MeSH Browser
• Hydroxyl Radical MeSH
• Copper MeSH
• Plant Oils * MeSH
• Olive Oil MeSH
• Hydrogen Peroxide MeSH
• Industrial Waste MeSH
• Free Radicals MeSH
• Iron MeSH

Neem hull waste (containing a high amount of lignin and other phenolic compounds) was used for lignin peroxidase production by Phanerochaete chrysosporum under solid-state fermentation conditions. Maximum decolorization achieved by partially purified lignin peroxidase was 80% for Porocion Brilliant Blue HGR, 83 for Ranocid Fast Blue, 70 for Acid Red 119 and 61 for Navidol Fast Black MSRL. The effects of different concentrations of veratryl alcohol, hydrogen peroxide, enzyme and dye on the efficiency of decolorization have been investigated. Maximum decolorization efficiency was observed at 0.2 and 0.4 mmol/L hydrogen peroxide, 2.5 mmol/L veratryl alcohol and pH 5.0 after a 1-h reaction, using 50 ppm of dyes and 9.96 mkat/L of enzyme.

• MeSH
• Azadirachta MeSH
• Color MeSH
• Coloring Agents metabolism   MeSH
• Benzyl Alcohols metabolism   MeSH
• Biodegradation, Environmental MeSH
• Bioreactors MeSH
• Hydrogen-Ion Concentration MeSH
• Culture Media MeSH
• Lignin metabolism   MeSH
• Peroxidases metabolism   MeSH
• Phanerochaete enzymology   MeSH
• Substrate Specificity MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Coloring Agents MeSH
• Benzyl Alcohols MeSH
• Culture Media MeSH
• lignin peroxidase MeSH Browser
• Lignin MeSH
• manganese peroxidase MeSH Browser
• Peroxidases MeSH
• veratryl alcohol MeSH Browser

Antifungal activity of new copper(II) complexes of 2-methylthionicotinate (2-MeSNic) of the composition Cu(2-MeSNic)2(MeNia)(2).4H2O (where MeNia is N-methylnicotinamide), and Cu(2-MeSNic)2(Nia)(2).2H2O (where Nia is nicotinamide) and Cu(2-MeSNic)2L2 (where L is isonicotinamide, iNia, or ethyl nicotinate, EtNic) were tested on various strains of filamentous fungi by the macrodilution method. Most sensitive against copper(II) adducts with bioactive ligands were Rhizopus oryzae and Microsporum gypseum (IC50 1.5-2.3 mmol/L). The adducts with Nia, MeNia and EtNic at 5 mmol/L induced morphological changes in growing hyphae of Botrytis cinerea, mainly their intensive branching attached to release of cytoplasm with partial growth inhibition. Inhibition of sporulation (> 90%) of Alternaria alternata by Cu(2-MeSNic)2.H2O was observed as a change in the color of the colonies. The highest resistance was marked by B. cinerea and Fusarium moniliforme (average IC50 values 4.25 and 3.13 mmol/L, respectively). The presence of all bioactive ligands in copper(II) complexes caused an increase in the inhibition effect against model fungi (except significant inhibition activity of EtNic on R. oryzae).

• MeSH
• Alternaria drug effects   MeSH
• Antifungal Agents chemistry   pharmacology   MeSH
• Fusarium drug effects   MeSH
• Copper chemistry   pharmacology   MeSH
• Microbial Sensitivity Tests MeSH
• Organometallic Compounds pharmacology   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Antifungal Agents MeSH
• Copper MeSH
• Organometallic Compounds MeSH

Biological properties of new copper(II) complexes of 2-methylthionicotinate (2-MeSNic) of composition Cu(2-MeSNic)2(MeNia)(2).4H2O (where MeNia is N-methyl-nicotinamide), Cu(2-MeSNic)2(Nia)(2).2H2O (where Nia is nicotinamide) and Cu(2-MeSNic)2L2 (where L is isonicotinamide (iNia) or ethyl nicotinate (EtNic)) are reported. Gram(-)-bacteria (Escherichia coli) are more resistant against Cu(II) complexes than Gram(+)-bacteria (Staphylococcus aureus)--significant antistaphylococcal activity was found with Cu(2-MeSNic)2(MeNia)(2).4H2O (IC50 1.3 mmol/L). Candida parapsilosis was most inhibited by Cu(2-MeSNic)2.H2O and Cu(2-MeSNic)2(MeNia)(2).4H2O (IC50 1.4 mmol/L and 1.5 mmol/L, respectively). Biosynthesis of nucleic acids influenced by Cu(2-MeSNic)2(Nia)(2).2H2O indicated by incorporation of 14C-adenine (IC50(Ade) 0.31 mmol/L) is more sensitive than biosynthesis of proteins indicated by incorporation of 14C-leucine (IC50(Leu) 9.94 mmol/L). Cu(II) complexes with expressed antimicrobial activity showed no mutagenic activity.

• MeSH
• Anti-Bacterial Agents MeSH
• Anti-Infective Agents pharmacology   MeSH
• Candida drug effects   MeSH
• Enterobacteriaceae drug effects   MeSH
• Ligands MeSH
• Copper pharmacology   MeSH
• Microbial Sensitivity Tests MeSH
• Organometallic Compounds pharmacology   MeSH
• Staphylococcus aureus drug effects   MeSH
• Dose-Response Relationship, Drug MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH
• Anti-Infective Agents MeSH
• Ligands MeSH
• Copper MeSH
• Organometallic Compounds MeSH