Interleukin-1α (IL-1α) is a dual-function proinflammatory mediator. In addition to its role in the canonical IL-1 signaling pathway, which employs membrane-bound receptors, a growing body of evidence shows that IL-1α has some additional intracellular functions. We identified the interaction of IL-1α with the tumor suppressor p53 in the nuclei and cytoplasm of both malignant and noncancerous mammalian cell lines using immunoprecipitation and the in situ proximity ligation assay (PLA). This interaction was enhanced by treatment with the antineoplastic drug etoposide, which suggests a role for the IL-1α•p53 interaction in genotoxic stress.

• MeSH
• cytoplazma metabolismus   MeSH
• dvouřetězcové zlomy DNA MeSH
• fluorescenční mikroskopie MeSH
• HeLa buňky MeSH
• imunoprecipitace MeSH
• interleukin-1alfa genetika   metabolismus   MeSH
• lidé MeSH
• nádorový supresorový protein p53 genetika   metabolismus   MeSH
• poškození DNA genetika   fyziologie   MeSH
• western blotting MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• interleukin-1alfa MeSH
• nádorový supresorový protein p53 MeSH

There are numerous data suggesting that two key steps in gene expression-transcription and splicing influence each other closely. For a long time it was known that chromatin modifications regulate transcription, but only recently it was shown that chromatin and histone modifications play a significant role in pre-mRNA splicing. Here we summarize interactions between splicing machinery and chromatin and discuss their potential functional significance. We focus mainly on histone acetylation and methylation and potential mechanisms of their role in splicing. It seems that whereas histone acetylation acts mainly by alterating the transcription rate, histone methylation can also influence splicing directly by recruiting various splicing components.

• Klíčová slova
• alternative splicing, chromatin, exon, histone acetylation, histone methylation, nucleosome, snRNP, transcription,
• MeSH
• chromatin genetika   metabolismus   MeSH
• genetická transkripce genetika   MeSH
• lidé MeSH
• nukleoproteiny metabolismus   MeSH
• prekurzory RNA genetika   MeSH
• sestřih RNA * MeSH
• transkripční faktory metabolismus   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• chromatin MeSH
• nukleoproteiny MeSH
• prekurzory RNA MeSH
• transkripční faktory MeSH