Platelet concentrates and especially their further product platelet lysate, are widely used as a replacement for cell culturing. Platelets contain a broad spectrum of growth factors and bioactive molecules that affect cellular fate. However, the cellular response to individual components of the human platelet concentrate is still unclear. The aim of this study was to observe cellular behavior according to the individual components of platelet concentrates. The bioactive molecule content was determined. The cells were supplemented with a medium containing 8% (v/v) of platelet proteins in plasma, pure platelet proteins in deionized water, and pure plasma. The results showed a higher concentration of fibrinogen, albumin, insulin growth factor I (IGF-1), keratinocyte growth factor (KGF), and hepatocyte growth factor (HGF), in the groups containing plasma. On the other hand, chemokine RANTES and platelet-derived growth factor bb (PDGF-bb), were higher in the groups containing platelet proteins. The groups containing both plasma and plasma proteins showed the most pronounced proliferation and viability of mesenchymal stem cells and fibroblasts. The platelet proteins alone were not sufficient to provide optimal cell growth and viability. A synergic effect of platelet proteins and plasma was observed. The data indicated the importance of plasma in platelet lysate for cell growth.

• Klíčová slova
• fibroblasts, mesenchymal stem cells, plasma, platelets,
• MeSH
• albuminy MeSH
• becaplermin metabolismus   MeSH
• buněčné kultury metody   MeSH
• chemokiny metabolismus   MeSH
• fibrinogen metabolismus   MeSH
• fibroblastový růstový faktor 7 MeSH
• fibroblasty metabolismus   MeSH
• hepatocytární růstový faktor MeSH
• insulinu podobný růstový faktor I MeSH
• krevní plazma chemie   MeSH
• kultivační média chemie   MeSH
• lidé MeSH
• mezenchymální kmenové buňky metabolismus   MeSH
• plazma bohatá na destičky metabolismus   MeSH
• proliferace buněk účinky léků   MeSH
• protoonkogenní proteiny c-sis metabolismus   MeSH
• trombocyty chemie   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• albuminy MeSH
• becaplermin MeSH
• chemokiny MeSH
• fibrinogen MeSH
• fibroblastový růstový faktor 7 MeSH
• hepatocytární růstový faktor MeSH
• insulinu podobný růstový faktor I MeSH
• kultivační média MeSH
• protoonkogenní proteiny c-sis MeSH