Tmprss6-mutated mask mice display iron deficiency anemia and high expression of hepcidin. The aim of the study was to determine the effect of erythropoietin administration on proteins participating in the control of iron homeostasis in the liver and spleen in C57BL/6 and mask mice. Administration of erythropoietin for four days at 50 IU/mouse/day increased hemoglobin and hematocrit in C57BL/6 mice, no such increase was seen in mask mice. Erythropoietin administration decreased hepcidin expression in C57BL/6 mice, but not in mask mice. Erythropoietin treatment significantly increased the spleen size in both C57BL/6 and mask mice. Furthermore, erythropoietin administration increased splenic Fam132b, Fam132a and Tfr2 mRNA content. At the protein level, erythropoietin increased the amount of splenic erythroferrone and transferrin receptor 2 both in C57BL/6 and mask mice. Splenic ferroportin content was decreased in erythropoietin-treated mask mice in comparison with erythropoietin-treated C57BL/6 mice. In mask mice, the amount of liver hemojuvelin was decreased in comparison with C57BL/6 mice. The pattern of hemojuvelin cleavage was different between C57BL/6 and mask mice: In both groups, a main hemojuvelin band was detected at approximately 52 kDa; in C57BL/6 mice, a minor cleaved band was seen at 47 kDa. In mask mice, the 47 kDa band was absent, but additional minor bands were detected at approximately 45 kDa and 48 kDa. The results provide support for the interaction between TMPRSS6 and hemojuvelin in vivo; they also suggest that hemojuvelin could be cleaved by another as yet unknown protease in the absence of functional TMPRSS6. The lack of effect of erythropoietin on hepcidin expression in mask mice can not be explained by changes in erythroferrone synthesis, as splenic erythroferrone content increased after erythropoietin administration in both C57BL/6 and mask mice.

• MeSH
• cytokiny genetika   metabolismus   MeSH
• erythropoetin genetika   farmakologie   MeSH
• GPI-vázané proteiny MeSH
• hepcidiny genetika   metabolismus   MeSH
• játra metabolismus   patologie   MeSH
• membránové proteiny genetika   metabolismus   MeSH
• mutantní kmeny myší MeSH
• myši MeSH
• protein hemochromatózy MeSH
• proteiny vázající RNA genetika   metabolismus   MeSH
• regulace genové exprese účinky léků   genetika   MeSH
• serinové endopeptidasy genetika   metabolismus   MeSH
• slezina metabolismus   patologie   MeSH
• svalové proteiny genetika   metabolismus   MeSH
• velikost orgánu účinky léků   genetika   MeSH
• železo metabolismus   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• Ankrd17 protein, mouse MeSH Prohlížeč
• cytokiny MeSH
• Erfe protein, mouse MeSH Prohlížeč
• erythropoetin MeSH
• GPI-vázané proteiny MeSH
• Hamp protein, mouse MeSH Prohlížeč
• hepcidiny MeSH
• HJV protein, mouse MeSH Prohlížeč
• matriptase 2 MeSH Prohlížeč
• membránové proteiny MeSH
• protein hemochromatózy MeSH
• proteiny vázající RNA MeSH
• serinové endopeptidasy MeSH
• svalové proteiny MeSH
• železo MeSH