The growth adaptability to increasing concentration of the biocide 2-phenoxyethanol (PE) was determined in Pseudomonas aeruginosa PAO1 (P.a.) as part of efforts to understand and control the biocide tolerance and its effect on cross-resistance to other biocides and resistance to antibiotics. After repeated subculturing in media containing increasing sub-minimum-inhibitory PE concentration, P.a. exhibited an adaptive resistance indicated by two-fold increase in MIC at the 10th passage. The resistance was stable and remained after passaging the strain in further 7 successive passages in PE-free growth media. The strain showed cross-resistance towards dissimilar biocides and displayed increased susceptibility to antibiotics, which was not influenced by the presence of the efflux inhibitor 'carbonyl cyanide m-chlorophenyl hydrazone'. Outer membranes of adapted strain showed altered protein profile when examined by SDS-PAGE.

• MeSH
• antibakteriální látky farmakologie   MeSH
• biologická adaptace MeSH
• dezinficiencia metabolismus   toxicita   MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• ethylenglykoly metabolismus   toxicita   MeSH
• mikrobiální testy citlivosti MeSH
• proteiny vnější bakteriální membrány analýza   MeSH
• proteom analýza   MeSH
• Pseudomonas aeruginosa fyziologie   MeSH
• sériové pasážování MeSH
• tolerance léku * MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antibakteriální látky MeSH
• dezinficiencia MeSH
• ethylenglykoly MeSH
• phenoxyethanol MeSH Prohlížeč
• proteiny vnější bakteriální membrány MeSH
• proteom MeSH