Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans. Here, we identify Mte1 as a novel interactor of the Mph1 helicase in Saccharomyces cerevisiae. In vitro, Mte1 (Mph1-associated telomere maintenance protein 1) binds directly to DNA with a preference for branched molecules such as D loops and fork structures. In addition, Mte1 stimulates the helicase and fork regression activities of Mph1 while inhibiting the ability of Mph1 to dissociate recombination intermediates. Deletion of MTE1 reduces crossover recombination and suppresses the sensitivity of mph1Δ mutant cells to replication stress. Mph1 and Mte1 interdependently colocalize at DNA damage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres. Taken together, our data indicate that Mte1 plays a role in regulation of crossover recombination, response to replication stress, and telomere maintenance.

• Klíčová slova
• DNA repair, Mph1, Mte1, genome integrity, homologous recombination, telomere maintenance,
• MeSH
• crossing over (genetika) genetika   MeSH
• DEAD-box RNA-helikasy genetika   metabolismus   MeSH
• delece genu MeSH
• fyziologický stres genetika   MeSH
• homeostáza telomer genetika   MeSH
• proteiny vázající telomery genetika   metabolismus   MeSH
• Saccharomyces cerevisiae - proteiny genetika   metabolismus   MeSH
• Saccharomyces cerevisiae genetika   metabolismus   MeSH
• transport proteinů MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DEAD-box RNA-helikasy MeSH
• MPH1 protein, S cerevisiae MeSH Prohlížeč
• Mte1 protein, S cerevisiae MeSH Prohlížeč
• proteiny vázající telomery MeSH
• Saccharomyces cerevisiae - proteiny MeSH