Secreted aspartyl proteinase (Sap) distribution among different C. albicans isolates was determined using SAP-specific primers in polymerase chain reaction (PCR) assay. SAP1, SAP2, and SAP3 were detected in 13 of 40 (32.5%), SAP4 in 38/40 (95%), SAP5 were detected in 30/40 (75%), SAP6 in 23/40 (57.5%) of C. albicans strains isolated from blood cultures. SAP1-SAP3 were detected in 37 of 40 (92.5%), SAP4 were detected in 3/40 (7.5%), SAP5 in 3/40 (7.5%), SAP6 in 5/40 (12.5%) of C. albicans strains isolated from vaginal swab cultures. Sap1, Sap2 and Sap3 isoenzymes were found to be related to the vaginopathic potential of C. albicans; Sap4, Sap5 and Sap6 isoenzymes were found to be correlated with systemic infections.

• MeSH
• aspartátové endopeptidasy analýza   genetika   MeSH
• Candida albicans enzymologie   genetika   izolace a purifikace   MeSH
• DNA fungální analýza   MeSH
• fungální proteiny analýza   genetika   MeSH
• geny hub MeSH
• izoenzymy antagonisté a inhibitory   genetika   MeSH
• kandidóza mikrobiologie   MeSH
• krev mikrobiologie   MeSH
• lidé MeSH
• polymerázová řetězová reakce metody   MeSH
• vagina mikrobiologie   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• aspartátové endopeptidasy MeSH
• DNA fungální MeSH
• fungální proteiny MeSH
• izoenzymy MeSH
• SAP1 protein, Candida albicans MeSH Prohlížeč
• SAP2 protein, Candida MeSH Prohlížeč
• SAP3 protein, Candida albicans MeSH Prohlížeč
• SAP4 protein, Candida albicans MeSH Prohlížeč
• SAP5 protein, Candida albicans MeSH Prohlížeč

Production of secreted aspartate proteinases was determined in a set of 646 isolates of Candida and non-Candida yeast species collected from 465 patients of the University Hospital in Olomouc (Czechia) in the period 1995-2002, and Candida samples obtained from 64 healthy volunteers using solid media developed for this purpose. Using random amplified polymorphic DNA analysis (RAPD) 79 Candida isolates from blood were analyzed to show potential relationships between clustering of the fingerprints and extracellular proteolytic activity of these strains. C. albicans, C. tropicalis and C. parapsilosis possess always proteolytic activity while non-Candida species did not display any proteolysis. A tight relationship between fingerprints and extracellular proteolysis in the Candida isolates was not shown. A remarkable consistency between fingerprint clusters and proteolysis occurred in a subset of C. parapsilosis samples. Suboptimal pH of the growth medium was shown to facilitate the investigation of potential co-incidence of genotypic and phenotypic traits.

• MeSH
• aspartátové endopeptidasy fyziologie   MeSH
• Candida enzymologie   patogenita   MeSH
• faktory virulence fyziologie   MeSH
• fungální proteiny fyziologie   MeSH
• koncentrace vodíkových iontů MeSH
• lidé MeSH
• technika náhodné amplifikace polymorfní DNA MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• aspartátové endopeptidasy MeSH
• faktory virulence MeSH
• fungální proteiny MeSH