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Nejvíce citované: 13876303

6 citací v PubMed Filtry

Nejvíce citovaný článek - PubMed ID 13876303

Microbial transformation of steroids. XVII. Transformation of progesterone by various species and strains of Penicillium

Folia microbiologica. 1962 Mar ; 7 () : 121-5.

Folia Microbiol (Praha)
ISSN 0015-5632
Zdroj

Článek

Progesterone side-chain degradation by some species of Aspergillus flavus group

Folia microbiologica. 2000 ; 45 (3) : 243-7.

Folia Microbiol (Praha)
ISSN 0015-5632
Zdroj

Seventy isolates belonging to 6 species and one variety of A. flavus group were shown to degrade the progesterone side-chain to yield delta 4-androstene-3,17-dione and testosterone. The isolates of five species (A. flavo-furcatis, A. flavus, A. oryzae, A. parasiticus and A. tamarii) possessed enzyme systems catalyzing the opening of ring D and formed testololactone as final steroid metabolite in addition to their ability to produce the above mentioned two products. 11 beta-Hydroxy-delta 4-androstene-3,17-dione was formed by only A. flavus and A. tamarii while 11 beta-hydroxytestosterone was produced by A. flavo-furcatis, A. parasiticus and A. subolivaceus. The chromatographic resolution of the mixture products obtained (when the selective isolate of each species reacted with 1 g of progesterone) revealed that 60-75% of progesterone was converted into delta 4-androstene-3,17-dione (8-30%), testosterone (7-33%), testololactone (14-37%) and other products (3-40%). The most bioconversion activity was exhibited by A. oryzae, followed by A. parasiticus. The highest values of delta 4-androstene-3,17-dione (30% of added progesterone) and testosterone (33%) were formed by A. flavus var. columnaris while those of testololactone (37%) were produced by A. oryzae. A systematic variation could be observed between the different tested species of A. flavus group with respect to the transformation reactions of progesterone. Comparative biotransformation results showed that essential differences exist between the tested species in this group; this biochemical differentiation may supplement the morphological and other physiological criteria used in the identification of the different species in the A. flavus group.

MeSH
androsteny metabolismus MeSH
Aspergillus flavus klasifikace růst a vývoj metabolismus MeSH
mykologické určovací techniky MeSH
progesteron chemie metabolismus MeSH
spektrofotometrie infračervená MeSH
testolakton metabolismus MeSH
testosteron metabolismus MeSH
vysokoúčinná kapalinová chromatografie MeSH
Publikační typ
časopisecké články MeSH
Názvy látek
androsteny MeSH
progesteron MeSH
testolakton MeSH
testosteron MeSH

Článek

Steroid derivatives. LXXIX. Microbial 15 beta-hydroxylation of 17 alpha-acetoxy-16-methylene derivatives of the pregnane series by the action of Cunninghamella blakesleeana

Folia microbiologica. 1976 ; 21 (2) : 144-51.

Folia Microbiol (Praha)
ISSN 0015-5632
Zdroj

Hydroxylation of 17 alpha-acetoxy-6-chloro-16-methylene-4,6-pregnadiene-3,20-dione (Chlorosuperlutin, I) by Cunninghamella blakesleeana yielded a 15 beta-hydroxyderivative II. Analogous transformation of 17 alpha-acetoxy-16-methylene-4,6-pregnadiene-3,20-dione (Superlutin, IV) included a hydroxylation in position 15 beta and probably also in 11 beta with a concomitant reduction of the 6,7-double bond.