Photorhabdus asymbiotica is one of the three recognized species of the Photorhabdus genus, which consists of gram-negative bioluminescent bacteria belonging to the family Morganellaceae. These bacteria live in a symbiotic relationship with nematodes from the genus Heterorhabditis, together forming a complex that is highly pathogenic for insects. Unlike other Photorhabdus species, which are strictly entomopathogenic, P. asymbiotica is unique in its ability to act as an emerging human pathogen. Analysis of the P. asymbiotica genome identified a novel fucose-binding lectin designated PHL with a strong sequence similarity to the recently described P. luminescens lectin PLL. Recombinant PHL exhibited high affinity for fucosylated carbohydrates and the unusual disaccharide 3,6-O-Me2-Glcβ1-4(2,3-O-Me2)Rhaα-O-(p-C6H4)-OCH2CH2NH2 from Mycobacterium leprae. Based on its crystal structure, PHL forms a seven-bladed β-propeller assembling into a homo-dimer with an inter-subunit disulfide bridge. Investigating complexes with different ligands revealed the existence of two sets of binding sites per monomer-the first type prefers l-fucose and its derivatives, whereas the second type can bind d-galactose. Based on the sequence analysis, PHL could contain up to twelve binding sites per monomer. PHL was shown to interact with all types of red blood cells and insect haemocytes. Interestingly, PHL inhibited the production of reactive oxygen species induced by zymosan A in human blood and antimicrobial activity both in human blood, serum and insect haemolymph. Concurrently, PHL increased the constitutive level of oxidants in the blood and induced melanisation in haemolymph. Our results suggest that PHL might play a crucial role in the interaction of P. asymbiotica with both human and insect hosts.

• MeSH
• Bacterial Proteins genetics   immunology   MeSH
• Host-Pathogen Interactions immunology   MeSH
• Protein Conformation MeSH
• Crystallography, X-Ray MeSH
• Lectins chemistry   genetics   immunology   MeSH
• Humans MeSH
• Molecular Sequence Data MeSH
• Photorhabdus genetics   immunology   MeSH
• Surface Plasmon Resonance MeSH
• Base Sequence MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Bacterial Proteins MeSH
• fucose-binding lectin MeSH Browser
• Lectins MeSH

Over the years, researchers have used presumptively neutral molecular variation to infer the origins of current species' distributions in northern latitudes (especially Europe). However, several reported examples of genic and chromosomal replacements suggest that end-glacial colonizations of particular northern areas may have involved genetic input from different source populations at different times, coupled with competition and selection. We investigate the functional consequences of differences between two bank vole (Clethrionomys glareolus) haemoglobins deriving from different glacial refugia, one of which partially replaced the other in Britain during end-glacial climate warming. This allows us to examine their adaptive divergence and hence a possible role of selection in the replacement. We determine the amino acid substitution Ser52Cys in the major expressed β-globin gene as the allelic difference. We use structural modelling to reveal that the protein environment renders the 52Cys thiol a highly reactive functional group and we show its reactivity in vitro. We demonstrate that possessing the reactive thiol in haemoglobin increases the resistance of bank vole erythrocytes to oxidative stress. Our study thus provides striking evidence for physiological differences between products of genic variants that spread at the expense of one another during colonization of an area from different glacial refugia.

• Keywords
• adaptation, antioxidative capacity, climate change, cysteine, oxidative stress, redox,
• MeSH
• Arvicolinae classification   genetics   metabolism   MeSH
• Phylogeography MeSH
• Genetic Variation MeSH
• Hemoglobins chemistry   genetics   metabolism   MeSH
• Molecular Sequence Data MeSH
• Amino Acid Substitution MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• United Kingdom MeSH
• Names of Substances
• Hemoglobins MeSH

Natural bioluminescence of all recently accepted Photorhabdus species and subspecies type strains (bacterial symbionts of entomopathogenic nematodes) was measured using a commercial luminometer; optimum conditions for the measurement were described. Cultures emitted reliably measurable bioluminescence with characteristic level and kinetics for each strain. Bioluminescence of all strains was significantly higher at 37 than at 25 degrees C at the beginning of the measurement, no effect of bacterial concentration on the intensity of bioluminescence was observed. The technique can provide reliable and quick information for the determination of Photorhabdus taxons.

• MeSH
• Species Specificity MeSH
• Kinetics MeSH
• Luminescence MeSH
• Luminescent Measurements MeSH
• Photorhabdus classification   metabolism   MeSH
• Rhabditoidea microbiology   MeSH
• Temperature MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Comparative Study MeSH