The bla(PER-1) presence was sought by PCR in 289 ceftazidime resistant Gram-negative bacteria isolated at Dokuz Eylul University Hospital (Turkey) between 1998 and 2003. PER-1 production rates were 32.3, 33.9, 14.9 and 37.9% in the 1998-2000 period, 2001, 2002 and 2003, respectively. bla(PER-1) was detected in 46.2 and 35.9% of ceftazidime-resistant Pseudomonas aeruginosa and Acinetobacter baumannii isolates, respectively. ERIC-PCR results revealed that dissemination of two endemic clones for both P. aeruginosa (X and Y) and A. baumannii (A and B) was responsible for the high prevalence. Results of the conjugation tests and plasmid curing experiments suggested that bla(PER-1) was located on the chromosome in the representative strains. It was also shown for the first time that bla(PER-1) in a clinical isolate was associated with class-1 integron which could facilitate dissemination of bla(PER-1) among bacteria.

• MeSH
• Acinetobacter baumannii drug effects   enzymology   genetics   isolation & purification   MeSH
• Anti-Bacterial Agents pharmacology   MeSH
• beta-Lactamases genetics   MeSH
• beta-Lactam Resistance drug effects   genetics   MeSH
• Ceftazidime pharmacology   MeSH
• DNA Fingerprinting MeSH
• Acinetobacter Infections epidemiology   transmission   MeSH
• Integrons MeSH
• Humans MeSH
• Microbial Sensitivity Tests MeSH
• Multigene Family MeSH
• Hospitals, University MeSH
• Polymerase Chain Reaction methods   MeSH
• Gene Transfer, Horizontal MeSH
• Pseudomonas Infections epidemiology   transmission   MeSH
• Pseudomonas aeruginosa drug effects   enzymology   genetics   isolation & purification   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Turkey epidemiology   MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH
• beta-lactamase PER-1 MeSH Browser
• beta-Lactamases MeSH
• Ceftazidime MeSH