Intracellular trafficking of organelles, driven by kinesin-1 stepping along microtubules, underpins essential cellular processes. In absence of other proteins on the microtubule surface, kinesin-1 performs micron-long runs. Under crowding conditions, however, kinesin-1 motility is drastically impeded. It is thus unclear how kinesin-1 acts as an efficient transporter in intracellular environments. Here, we demonstrate that TRAK1 (Milton), an adaptor protein essential for mitochondrial trafficking, activates kinesin-1 and increases robustness of kinesin-1 stepping on crowded microtubule surfaces. Interaction with TRAK1 i) facilitates kinesin-1 navigation around obstacles, ii) increases the probability of kinesin-1 passing through cohesive islands of tau and iii) increases the run length of kinesin-1 in cell lysate. We explain the enhanced motility by the observed direct interaction of TRAK1 with microtubules, providing an additional anchor for the kinesin-1-TRAK1 complex. Furthermore, TRAK1 enables mitochondrial transport in vitro. We propose adaptor-mediated tethering as a mechanism regulating kinesin-1 motility in various cellular environments.

• MeSH
• adaptorové proteiny vezikulární transportní genetika   izolace a purifikace   metabolismus   MeSH
• fluorescenční mikroskopie MeSH
• kineziny genetika   izolace a purifikace   metabolismus   MeSH
• luminescentní proteiny genetika   metabolismus   MeSH
• mikrotubuly metabolismus   MeSH
• mitochondrie metabolismus   MeSH
• myši MeSH
• nádorové buněčné linie MeSH
• proteiny tau genetika   metabolismus   MeSH
• rekombinantní proteiny genetika   metabolismus   MeSH
• vnitřně neuspořádané proteiny genetika   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• adaptorové proteiny vezikulární transportní MeSH
• KIF5B protein, human MeSH Prohlížeč
• kineziny MeSH
• luminescentní proteiny MeSH
• proteiny tau MeSH
• rekombinantní proteiny MeSH
• TRAK1 protein, human MeSH Prohlížeč
• vnitřně neuspořádané proteiny MeSH