Relations between the diversity of the fliC gene conditioning flagellum protein in E. coli and the source of the strain origin are presented. The fliC genes have been identified and characterized in commensal E. coli derived from 10 healthy animal species living in Zoo Safari Park (Poland). The fliC gene was found in 150 strains by the PCR method. The amplifiedfliC products revealed single bands within the range 1.26-2.16 kbp. Forty restriction patterns (classed by restriction analysis with the use of RsaI (PCR-RFLP RsaI; R-types) were determined. The neighbor-joining method was employed to illustrate the distribution of the kinds of R-types. There are 3-8 various R-types of a diversified frequency of occurrence in strains. Application of PCR-RFLP RsaI permitted the identification of alleles of fliC genes characteristic for E. coli and the estimation of their diversity among the animal species. The transmission ways of E. coli fliC+ between organisms of different species were determined and confirmed the role of transmission and horizontal gene transfer in the generation of the allelic diversity of fliC gene in natural E. coli populations.

• MeSH
• alely MeSH
• Escherichia coli genetika   MeSH
• flagelin MeSH
• fylogeneze MeSH
• genetická variace genetika   MeSH
• infekce vyvolané Escherichia coli genetika   přenos   veterinární   MeSH
• polymorfismus délky restrikčních fragmentů MeSH
• přenos genů horizontální MeSH
• proteiny z Escherichia coli genetika   MeSH
• savci mikrobiologie   MeSH
• zvířata v ZOO mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• flagelin MeSH
• FliC protein, E coli MeSH Prohlížeč
• proteiny z Escherichia coli MeSH

Inhibitory effect of various lactobacilli against pathogenic strains of E. coli in model system Caco2 cells was determined by enumerating the number of adhering E. coli after pre-incubation (exclusion), post-incubation (displacement) or co-incubation (competition) with lactobacilli. Porcine E. coli strain F107 (F18ab, Stx2v) in the competition assay with porcine lactobacillus strain P10 gave bacterial counts 7.25 (log CFU per well); in the exclusion test it was only 7.05 while in displacement test it reached 7.29. The lowest E. coli counts adhering to Caco-2 cells were in exclusion assay (pre-incubation, Lactobacillus inoculated as the first). Pre-treatment of E. coli with our lactobacilli strains reduced the cultivable E. coli numbers.

• MeSH
• bakteriální adheze fyziologie   MeSH
• Caco-2 buňky MeSH
• Escherichia coli fyziologie   MeSH
• Lactobacillus metabolismus   MeSH
• lidé MeSH
• počet mikrobiálních kolonií MeSH
• prasata mikrobiologie   MeSH
• probiotika farmakologie   MeSH
• střevní sliznice mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

One-hundred sixty Escherichia coli isolates obtained from piglets with diarrhea from different parts of Slovakia were examined for the presence of genes coding for F4, F5, F6 and F41 fimbrial adhesins, and hemolytic activity. According to polymerase chain reaction tests 74 (46%) E. coli isolates were positive for primers that detected genes coding for fimbrial adhesins. Of these 74 isolates, 64 were positive for genes encoding for F4+, four for F5+, five for F6+, and one for both F41+ and F5+ adhesins.

• MeSH
• adheziny Escherichia coli genetika   MeSH
• antigeny bakteriální genetika   MeSH
• bakteriální adheziny genetika   MeSH
• bakteriální toxiny biosyntéza   MeSH
• DNA bakterií analýza   genetika   MeSH
• Escherichia coli genetika   izolace a purifikace   MeSH
• genotyp MeSH
• hemolyziny biosyntéza   MeSH
• infekce vyvolané Escherichia coli mikrobiologie   veterinární   MeSH
• nemoci prasat mikrobiologie   MeSH
• polymerázová řetězová reakce MeSH
• prasata mikrobiologie   MeSH
• proteiny z Escherichia coli genetika   MeSH
• průjem mikrobiologie   veterinární   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Slovenská republika MeSH
• Názvy látek
• adheziny Escherichia coli MeSH
• antigeny bakteriální MeSH
• bakteriální adheziny MeSH
• bakteriální toxiny MeSH
• DNA bakterií MeSH
• F41 antigen, E coli MeSH Prohlížeč
• hemolyziny MeSH
• proteiny z Escherichia coli MeSH