Nejvíce citovaný článek - PubMed ID 16255818
Stimulation of innate immunity in newborn kids against Cryptosporidium parvum infection-challenge by intranasal/per-oral administration of liposomal formulation of N-L18-norAbu-GMDP adjuvant
PURPOSE: The aim of this work was to demonstrate an immunostimulatory and adjuvant effect of new apyrogenic lipophilic derivatives of norAbuMDP and norAbuGMDP formulated in nanoliposomes. METHODS: Nanoliposomes and metallochelating nanoliposomes were prepared by lipid film hydration and extrusion methods. The structure of the liposomal formulation was studied by electron microscopy, AF microscopy, and dynamic light scattering. Sublethal and lethal γ-irradiation mice models were used to demonstrate stimulation of innate immune system. Recombinant Hsp90 antigen (Candida albicans) bound onto metallochelating nanoliposomes was used for immunisation of mice to demonstrate adjuvant activities of tested compounds. RESULTS: Safety and stimulation of innate and adaptive immunity were demonstrated on rabbits and mice. The liposomal formulation of norAbuMDP/GMDP was apyrogenic in rabbit test and lacking any side effect in vivo. Recovery of bone marrow after sublethal γ-irradiation as well as increased survival of mice after lethal irradiation was demonstrated. Enhancement of specific immune response was demonstrated for some derivatives incorporated in metallochelating nanoliposomes with recombinant Hsp90 protein antigen. CONCLUSIONS: Liposomal formulations of new lipophilic derivatives of norAbuMDP/GMDP proved themselves as promising adjuvants for recombinant vaccines as well as immunomodulators for stimulation of innate immunity and bone-marrow recovery after chemo/radio therapy of cancer.
- MeSH
- acetylmuramyl-alanyl-isoglutamin aplikace a dávkování analogy a deriváty chemie farmakologie terapeutické užití MeSH
- adaptivní imunita účinky léků MeSH
- adjuvancia imunologická aplikace a dávkování chemie farmakologie terapeutické užití MeSH
- analýza přežití MeSH
- antigeny fungální imunologie MeSH
- experimentální radiační poranění imunologie prevence a kontrola MeSH
- králíci MeSH
- liposomy MeSH
- mikroskopie atomárních sil MeSH
- mikroskopie elektronová rastrovací MeSH
- molekulární struktura MeSH
- myši inbrední ICR MeSH
- myši MeSH
- nanočástice MeSH
- nosiče léků chemie MeSH
- přirozená imunita účinky léků MeSH
- proteiny tepelného šoku HSP90 imunologie MeSH
- protilátky fungální krev MeSH
- rekombinantní proteiny imunologie MeSH
- transmisní elektronová mikroskopie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- acetylmuramyl-alanyl-isoglutamin MeSH
- adjuvancia imunologická MeSH
- antigeny fungální MeSH
- glucosaminylmuramyl-2-alanine-D-isoglutamine MeSH Prohlížeč
- liposomy MeSH
- N-acetylmuramyl-aminobutyryl-isoglutamine MeSH Prohlížeč
- nosiče léků MeSH
- proteiny tepelného šoku HSP90 MeSH
- protilátky fungální MeSH
- rekombinantní proteiny MeSH
