Nejvíce citovaný článek - PubMed ID 16678136
Structure and dynamics of the N-terminal loop of PsbQ from photosystem II of Spinacia oleracea
The extrinsic proteins of photosystem II of higher plants and green algae PsbO, PsbP, PsbQ, and PsbR are essential for stable oxygen production in the oxygen evolving center. In the available X-ray crystallographic structure of higher plant PsbQ residues S14-Y33 are missing. Building on the backbone NMR assignment of PsbQ, which includes this "missing link", we report the extended resonance assignment including side chain atoms. Based on nuclear Overhauser effect spectra a high resolution solution structure of PsbQ with a backbone RMSD of 0.81 Å was obtained from torsion angle dynamics. Within the N-terminal residues 1-45 the solution structure deviates significantly from the X-ray crystallographic one, while the four-helix bundle core found previously is confirmed. A short α-helix is observed in the solution structure at the location where a β-strand had been proposed in the earlier crystallographic study. NMR relaxation data and unrestrained molecular dynamics simulations corroborate that the N-terminal region behaves as a flexible tail with a persistent short local helical secondary structure, while no indications of forming a β-strand are found.
- Klíčová slova
- Spinacia oleracea, dynamic N-terminus, extrinsic photosynthetic protein, hydrogen bond dynamics, intrinsic disorder, solution structure,
- MeSH
- fotosystém II (proteinový komplex) chemie genetika metabolismus MeSH
- krystalografie rentgenová MeSH
- magnetická rezonanční spektroskopie metody MeSH
- rekombinantní proteiny chemie metabolismus MeSH
- rostlinné proteiny chemie genetika metabolismus MeSH
- roztoky MeSH
- sekundární struktura proteinů * MeSH
- sekvence aminokyselin MeSH
- simulace molekulární dynamiky * MeSH
- Spinacia oleracea genetika metabolismus MeSH
- terciární struktura proteinů MeSH
- termodynamika MeSH
- vazba proteinů MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fotosystém II (proteinový komplex) MeSH
- rekombinantní proteiny MeSH
- rostlinné proteiny MeSH
- roztoky MeSH
