Eleven strains of Lactobacillus collected in the Culture Collection of Dairy Microorganisms (CCDM) were evaluated for selected probiotic properties such as survival in gastrointestinal fluids, antimicrobial activity, and competition with non-toxigenic Escherichia coli O157:H7 for adhesion on Caco-2 cells. The viable count of lactobacilli was reduced during 3-h incubation in gastric fluid followed by 3-h incubation in intestinal fluid. All strains showed antimicrobial activity and the three most effective strains inhibited the growth of at least 16 indicator strains. Antimicrobial metabolites of seven strains active against Lactobacillus and Clostridium indicator strains were found to be sensitive to proteinase K and trypsin, which indicates their proteinaceous nature. The degree of competitive inhibition of non-toxigenic E. coli O157:H7 adhesion on the surface of Caco-2 cells was strain-dependent. A significant decrease (P < 0.05) in the number of non-toxigenic E. coli O157:H7 adhering to Caco-2 cells was observed with all lactobacilli. Three strains were selected for additional studies of antimicrobial activity, i.e., Lactobacillus gasseri CCDM 215, Lactobacillus acidophilus CCDM 149, and Lactobacillus helveticus CCDM 82.

• MeSH
• antibióza * MeSH
• antiinfekční látky metabolismus   MeSH
• bakteriální adheze MeSH
• Caco-2 buňky MeSH
• epitelové buňky mikrobiologie   MeSH
• Escherichia coli O157 fyziologie   MeSH
• Lactobacillus růst a vývoj   fyziologie   MeSH
• lidé MeSH
• mikrobiální viabilita účinky léků   MeSH
• probiotika farmakologie   MeSH
• žaludeční šťáva mikrobiologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antiinfekční látky MeSH

Twelve lactobacilli isolates from mucosa of 3-5-week-old weaned pigs were found to exert good antimicrobial activity against common porcine pathogens (S. aureus, B. cereus, E. coli, C. perfringens). Two of them produced in addition to lactic acid also considerable amounts of acetic acid, and 6 of them produced hydrogen peroxide and metabolites other than organic acids. Isolates 4/26 and 2/25 (identified as L. crispatus or L. amylovorus) were inhibitory against most strains of S. aureus, B. cereus and E. coli, and especially the strain 4/26 survived well in simulated gastric and intestinal juice. Diarrhea-causing E. coli O8K88H9 Ent(+) was successfully inhibited by the growing culture as well as by the catalase-treated and neutralized supernatant of L. reuteri 12/26. Mucin degradation and multiple resistance to antibiotics were not observed.

• MeSH
• antibiotická rezistence MeSH
• Bacillus cereus účinky léků   MeSH
• Clostridium perfringens účinky léků   MeSH
• Escherichia coli účinky léků   MeSH
• feces mikrobiologie   MeSH
• ileum mikrobiologie   MeSH
• kultivační média speciální farmakologie   MeSH
• kultivační média chemie   farmakologie   MeSH
• kyselina mléčná farmakologie   MeSH
• Lactobacillus účinky léků   izolace a purifikace   metabolismus   MeSH
• muciny metabolismus   MeSH
• nemoci prasat prevence a kontrola   MeSH
• odstavení MeSH
• peroxid vodíku metabolismus   MeSH
• přirozená imunita MeSH
• probiotika MeSH
• Staphylococcus aureus účinky léků   MeSH
• střevní sliznice mikrobiologie   MeSH
• Sus scrofa mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• kultivační média speciální MeSH
• kultivační média MeSH
• kyselina mléčná MeSH
• muciny MeSH
• peroxid vodíku MeSH