Molecular cloning, nucleotide sequencing, and characterization of the flaA gene from additional isolates of urease-positive thermophilic Campylobacter (UPTC) were performed. These isolates were obtained from the natural environment in Northern Ireland (n = 9 from mussels) and in England (n = 1 from sea water). All isolates carried the shorter flaA gene, [open reading frames (ORFs), 1,461 to 1,503 base pairs], without any internal termination codons, and did not carry any flaA pseudogenes. The UPTC isolates were well discriminated by the neighbor joining (NJ) phylogenetic tree constructed based on the putative flaA genes ORFs nucleotide sequence information. In addition, the NJ tree constructed based on the flaA-short variable region sequence information discriminated the Campylobacter lari isolates with a similar degree of discrimination power.

• MeSH
• Campylobacter lari klasifikace   genetika   izolace a purifikace   metabolismus   MeSH
• Campylobacter klasifikace   genetika   izolace a purifikace   metabolismus   MeSH
• flagelin chemie   genetika   metabolismus   MeSH
• fylogeneze MeSH
• klonování DNA MeSH
• mlži mikrobiologie   MeSH
• molekulární sekvence - údaje MeSH
• mořská voda mikrobiologie   MeSH
• otevřené čtecí rámce MeSH
• polymerázová řetězová reakce MeSH
• rekombinantní proteiny chemie   genetika   metabolismus   MeSH
• sekvence aminokyselin MeSH
• sekvenční analýza DNA metody   MeSH
• sekvenční seřazení MeSH
• ureasa metabolismus   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Anglie MeSH
• Severní Irsko MeSH
• Názvy látek
• flaA protein, bacteria MeSH Prohlížeč
• flagelin MeSH
• rekombinantní proteiny MeSH
• ureasa MeSH

We aimed to clarify if the thermophilic Campylobacter lari organisms including urease-negative (UN) C. lari and urease-positive thermophilic Campylobacter (UPTC) can be differentiated at the species and/or subspecies levels by employing the full-length flaA gene and flaA short variable region (SVR) nucleotide sequence information or not. Thermophilic Campylobacter isolates (n = 45) including UN C. lari (n = 17), UPTC (n = 18), and Campylobacter jejuni (n = 10) were well discriminated at the isolate level by the unweighted pair group method using arithmetic means analysis and neighbor joining procedures constructed based on the full-length flaA gene and flaA SVR nucleotide sequence information. Thus, these procedures may possibly be useful for epidemimological studies for C. lari and C. jejuni.

• MeSH
• Campylobacter jejuni klasifikace   genetika   izolace a purifikace   MeSH
• Campylobacter lari klasifikace   genetika   izolace a purifikace   MeSH
• DNA bakterií chemie   genetika   MeSH
• flagelin genetika   MeSH
• genotyp MeSH
• lidé MeSH
• molekulární sekvence - údaje MeSH
• polymorfismus genetický * MeSH
• sekvenční analýza DNA MeSH
• shluková analýza MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• DNA bakterií MeSH
• flaA protein, bacteria MeSH Prohlížeč
• flagelin MeSH

The Campylobacter species strains (n = 42; isolated from clinical samples and deposited in Czech National Collection of Type Cultures, Prague) originally phenotypically (and biochemically) identified as Campylobacter jejuni were re-classified using molecular biological and mass spectrometric methods. Whole-cell MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) separated the isolates into two genetically related strains--C. jejuni (n = 26) and C. coli (n = 16) and, moreover, distinguished the intimate details in the group of tested strains. It also made it possible to create the MALDI-TOF MS dendrogram; similarly, the spectral characteristics were used for the 3D cluster analysis. Polymerase chain reaction (PCR) confirmed the results obtained by mass spectrometry. Both methods (PCR and MALDI-TOF MS) gave the same results which supports their suitability in the rapid and accurate Campylobacter-species determination.

• MeSH
• Campylobacter coli klasifikace   genetika   MeSH
• Campylobacter jejuni klasifikace   genetika   MeSH
• DNA bakterií analýza   izolace a purifikace   MeSH
• druhová specificita MeSH
• feces mikrobiologie   MeSH
• kočky MeSH
• lidé MeSH
• polymerázová řetězová reakce * MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice metody   MeSH
• techniky typizace bakterií * MeSH
• zvířata MeSH
• Check Tag
• kočky MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• Názvy látek
• DNA bakterií MeSH