A routine diagnostic procedure of cryptococcal meningitis using Alcian Blue and Nuclear Fast Red staining is described in a group of 16 patients. Cerebrospinal fluid findings, including clinical cytology, routine biochemistry and protein fractions, are presented.

• MeSH
• Staining and Labeling methods   MeSH
• Cryptococcus neoformans isolation & purification   MeSH
• Meningitis, Cryptococcal diagnosis   microbiology   MeSH
• Humans MeSH
• Microbiological Techniques methods   MeSH
• Microscopy methods   MeSH
• Cerebrospinal Fluid microbiology   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH

The levels of prealbumin (PAB, transthyretin) were determined and evaluated in the cerebrospinal fluid (CSF) and serum in various subgroups of the multiple sclerosis (MS) patients. In severely disabled patients, serum PAB was elevated more frequently. CSF and serum PAB concentrations were higher in treated than in nontreated patients; the values above the upper reference limits were also more frequently found in treated patients. PAB index showed a tendency to decrease during the course of the disease. The routine determination of PAB in CSF and serum is, therefore, recommended to be recognized in MS patients as a substantial clinical value and, thus, be comprised, including also immunoglobulins and other parameters, into the spectrum of characteristics in Protein Panel.

• MeSH
• Humans MeSH
• Prealbumin analysis   cerebrospinal fluid   MeSH
• Multiple Sclerosis blood   cerebrospinal fluid   pathology   MeSH
• Severity of Illness Index MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Publication type
• Journal Article MeSH
• Comparative Study MeSH
• Names of Substances
• Prealbumin MeSH

Sixty-five samples of cerebrospinal fluid (CSF) were evaluated using an automated cytoflow method with the CD-Sapphire hematology analyzer in order to investigate possible relationships between cell population patterns and diagnostic groups and better understand the biology of neurological disease. A basic panel of CD markers, including CD3/4/8/19/138/HLA-DR, was used to analyze CSF samples from clinical and laboratory confirmed cases of multiple sclerosis, neuroborreliosis, viral and bacterial neuroinfective diseases, malignant infiltrations of meninges and scavenger macrophagic reactions of the central nervous system. The principles of immune response and the contribution of cytological 'disease-related patterns' for these nosological entities are described. The distinct patterns of lymphocyte subpopulations in neuroborreliosis appear to be characteristic and could possibly serve as diagnostic indicators. Further verification and research will be necessary to clarify the significance and nature of CD4+ CD8+ positive subset in cerebrospinal fluid.

• MeSH
• Antigens, CD cerebrospinal fluid   immunology   MeSH
• Encephalitis, Viral immunology   MeSH
• Humans MeSH
• Lyme Neuroborreliosis immunology   MeSH
• Meningitis, Viral immunology   MeSH
• Antibodies, Monoclonal immunology   MeSH
• Cerebrospinal Fluid cytology   immunology   MeSH
• Central Nervous System Diseases cerebrospinal fluid   diagnosis   immunology   MeSH
• Lymphocyte Subsets cytology   immunology   MeSH
• Flow Cytometry methods   MeSH
• Multiple Sclerosis immunology   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Evaluation Study MeSH
• Names of Substances
• Antigens, CD MeSH
• Antibodies, Monoclonal MeSH