Although many noble metals are known for their antibacterial properties against the most common pathogens, such as Escherichia coli and Staphylococcus epidermidis, their effect on healthy cells can be toxic. For this reason, the choice of metals that preserve the antibacterial effect while being biocompatible with health cells is very important. This work aims to validate the effect of gold on the biocompatibility of Au/Ag nanowires, as assessed in our previous study. Polyethylene naphthalate (PEN) was treated with a KrF excimer laser to provide specific laser-induced periodic structures. Then, Au was deposited onto the modified PEN via a vacuum evaporation method. Atomic force microscopy and scanning electron microscopy revealed the dependence of the surface morphology on the incidence angle of the laser beam. A resazurin assay cytotoxicity test confirmed safety against healthy human cells and even cell proliferation was observed after 72 h of incubation. We have obtained satisfactory results, demonstrating that monometallic Au nanowires can be applied in biomedical applications and provide the biocompatibility of bimetallic Au/AgNWs.

• Klíčová slova
• biocompatibility, gold nanowires, laser-treatment, nanocomposites, periodic structures,
• MeSH
• antibakteriální látky farmakologie   MeSH
• Escherichia coli MeSH
• lasery MeSH
• lidé MeSH
• naftaleny MeSH
• nanodráty * chemie   MeSH
• polyethyleny MeSH
• zlato chemie   farmakologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• naftaleny MeSH
• poly(ethylene naphthalate) MeSH Prohlížeč
• polyethyleny MeSH
• zlato MeSH

As inflammation frequently occurs after the implantation of a medical device, biocompatible, antibacterial materials must be used. Polymer-metal nanocomposites are promising materials. Here we prepared enhanced polyethylene naphthalate (PEN) using surface modification techniques and investigated its suitability for biomedical applications. The PEN was modified by a KrF laser forming periodic ripple patterns with specific surface characteristics. Next, Au/Ag nanowires were deposited onto the patterned PEN using vacuum evaporation. Atomic force microscopy confirmed that the surface morphology of the modified PEN changed accordingly with the incidence angle of the laser beam. Energy-dispersive X-ray spectroscopy showed that the distribution of the selected metals was dependent on the evaporation technique. Our bimetallic nanowires appear to be promising antibacterial agents due to the presence of antibacterial noble metals. The antibacterial effect of the prepared Au/Ag nanowires against E. coli and S. epidermidis was demonstrated using 24 h incubation with a drop plate test. Moreover, a WST-1 cytotoxicity test that was performed to determine the toxicity of the nanowires showed that the materials could be considered non-toxic. Collectively, these results suggest that prepared Au/Ag nanostructures are effective, biocompatible surface coatings for use in medical devices.

• Klíčová slova
• antibacterial properties, bimetallic nanowires, biocompatibility, nanostructure, polymer, surface modification,
• Publikační typ
• časopisecké články MeSH

The study monitored in vitro early response of connective tissue cells and immunocompetent cells to enosseal implant materials coated by different blood components (serum, activated plasma, and plasma/platelets) to evaluate human osteoblast proliferation and synthetic activity and inflammatory response presented as a cytokine profile of peripheral blood mononuclear cells (PBMCs) under conditions imitating the situation upon implantation. The cells were cultivated on coated Ti-plasma-sprayed (Ti-PS), Ti-etched (Ti-Etch), Ti-hydroxyapatite (Ti-HA), and ZrO2 surfaces. The plasma/platelets coating supported osteoblast proliferation only on osteoconductive Ti-HA and Ti-Etch whereas activated plasma enhanced proliferation on all surfaces. Differentiation (BAP) and IL-8 production remained unchanged or decreased irrespective of the coating and surface; only the serum and plasma/platelets-coated ZrO2 exhibited higher BAP and IL-8 expression. RANKL production increased on serum and activated plasma coatings. PBMCs produced especially cytokines playing role in inflammatory phase of wound healing, that is, IL-6, GRO-α, GRO, ENA-78, IL-8, GM-CSF, EGF, and MCP-1. Cytokine profiles were comparable for all tested surfaces; only ENA-78, IL-8, GM-CSF, and MCP-1 expression depended on materials and coatings. The activated plasma coating led to uniformed surfaces and represented a favorable treatment especially for bioinert Ti-PS and ZrO2 whereas all coatings had no distinctive effect on bioactive Ti-HA and Ti-Etch.

• MeSH
• biokompatibilní potahované materiály škodlivé účinky   chemie   MeSH
• buněčné linie MeSH
• chemokin CCL2 metabolismus   MeSH
• chemokin CXCL1 metabolismus   MeSH
• chemokin CXCL5 metabolismus   MeSH
• cytokiny metabolismus   MeSH
• epidermální růstový faktor metabolismus   MeSH
• faktor stimulující granulocyto-makrofágové kolonie metabolismus   MeSH
• hojení ran účinky léků   MeSH
• interleukin-6 metabolismus   MeSH
• interleukin-8 metabolismus   MeSH
• leukocyty mononukleární účinky léků   metabolismus   MeSH
• lidé MeSH
• osteoblasty účinky léků   metabolismus   MeSH
• proliferace buněk účinky léků   MeSH
• titan škodlivé účinky   chemie   MeSH
• zánět metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• biokompatibilní potahované materiály MeSH
• chemokin CCL2 MeSH
• chemokin CXCL1 MeSH
• chemokin CXCL5 MeSH
• CXCL1 protein, human MeSH Prohlížeč
• CXCL5 protein, human MeSH Prohlížeč
• cytokiny MeSH
• epidermální růstový faktor MeSH
• faktor stimulující granulocyto-makrofágové kolonie MeSH
• interleukin-6 MeSH
• interleukin-8 MeSH
• titan MeSH