The presence of Anaplasma spp. and Borrelia burgdorferi sensu lato in rodents from Eastern Slovakia were followed by serological and molecular methods. The seroprevalence for Borrelia was detected in 16.6 %, for Anaplasmataceae (APT) in 13.2 % and co-occurrence of Borrelia and APT in 7.5 %. Out of 110 ear biopsies of rodents, 5 were B. afzelii-positive. Five biopsies tested positive with the Ehr521-Ehr747 primers amplifying all the members of the family APT. A. phagocytophilum was detected in 1.8 %, 2.7 % were infected with Anaplasma-like organisms. Co-occurrence of Borrelia and Anaplasma in ear biopsies was found in 1.8 %. The circulation of both Borrelia and Anaplasma in the region of Eastern Slovakia was confirmed.

• MeSH
• Anaplasmataceae genetics   immunology   isolation & purification   MeSH
• Arvicolinae microbiology   MeSH
• Borrelia burgdorferi Group isolation & purification   MeSH
• DNA, Bacterial genetics   isolation & purification   MeSH
• Enzyme-Linked Immunosorbent Assay * MeSH
• Anaplasmataceae Infections diagnosis   epidemiology   microbiology   veterinary   MeSH
• Lyme Disease diagnosis   epidemiology   microbiology   veterinary   MeSH
• Molecular Sequence Data MeSH
• Murinae microbiology   MeSH
• Mice MeSH
• Rodent Diseases diagnosis   epidemiology   microbiology   MeSH
• Polymerase Chain Reaction * MeSH
• Polymorphism, Restriction Fragment Length * MeSH
• Antibodies, Bacterial blood   MeSH
• Reproducibility of Results MeSH
• Sequence Alignment MeSH
• Seroepidemiologic Studies MeSH
• Disease Reservoirs MeSH
• Ear, External microbiology   MeSH
• Animals MeSH
• Check Tag
• Mice MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Slovakia epidemiology   MeSH
• Names of Substances
• DNA, Bacterial MeSH
• Antibodies, Bacterial MeSH

The genotype of Borrelia burgdorferi sensu lato was detected in 371 out of 1244 ticks. Borrelia determination was based on partial sequencing of the 16S rRNA gene and real-time polymerase chain reactions for identification and quantitation of ospA and recA genes. Different Borrelia spp. were identified; B. garinii in 40% ticks followed by B. afzelii (36.3%), B. burgdorferi sensu stricto (12.9%), B. valaisiana (3.5%), B. lusitaniae (0.8%), B. bissettii (0.5%) and B. miyamotoi-like (0.5%). Cultivation of 30 borrelia strains in BSK-H medium, among them B. valaisiana, B. bissettii-like and B. miyamotoi-like strains was unique in Czechia. Calibrated microfluidic-based quantification showed differences in the concentration of the nucleic acids and molar mass of the outer surface proteins of different Borrelia spp. with standard sensitivity and specificity and was helpful for their identification. The outer surface protein OspA was absent in B. miyamotoi-like and the OspB protein in B. valaisiana, B. lusitaniae and in three subtypes of B. garinii.

• MeSH
• Antigens, Surface chemistry   genetics   MeSH
• Bacterial Vaccines chemistry   genetics   MeSH
• Borrelia burgdorferi Group genetics   isolation & purification   MeSH
• DNA, Bacterial chemistry   genetics   MeSH
• Phylogeny MeSH
• Genetic Variation MeSH
• Ixodes microbiology   MeSH
• Lipoproteins chemistry   genetics   MeSH
• Microfluidic Analytical Techniques MeSH
• Molecular Sequence Data MeSH
• Polymerase Chain Reaction MeSH
• Bacterial Outer Membrane Proteins chemistry   genetics   MeSH
• Rec A Recombinases chemistry   genetics   MeSH
• RNA, Ribosomal, 16S chemistry   genetics   MeSH
• Base Sequence MeSH
• Sequence Alignment MeSH
• Animals MeSH
• Check Tag
• Male MeSH
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Antigens, Surface MeSH
• Bacterial Vaccines MeSH
• DNA, Bacterial MeSH
• Lipoproteins MeSH
• OspA protein MeSH Browser
• Bacterial Outer Membrane Proteins MeSH
• Rec A Recombinases MeSH
• RNA, Ribosomal, 16S MeSH