The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laboratory host Nicotiana tabacum cv. Petit Havana SR1 and in various cultivars of the natural host Solanum tuberosum by ELISA as well as by Western blots. The antibodies can be used for the detection of the whole strain spectrum of PVY by indirect plate trapped antigen ELISA and Western blot, but not by double antigen sandwich ELISA.

• MeSH
• ELISA MeSH
• králíci MeSH
• monoklonální protilátky biosyntéza   MeSH
• nemoci rostlin virologie   MeSH
• Potyvirus imunologie   MeSH
• protilátky virové biosyntéza   MeSH
• rekombinantní proteiny imunologie   MeSH
• Solanum tuberosum virologie   MeSH
• tabák virologie   MeSH
• virové plášťové proteiny imunologie   MeSH
• western blotting MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• coat protein, Potato virus Y MeSH Prohlížeč
• monoklonální protilátky MeSH
• protilátky virové MeSH
• rekombinantní proteiny MeSH
• virové plášťové proteiny MeSH