A selection of tert-butylhydroperoxide (tBOOH)-tolerant Candida albicans mutants showed increased tolerances to 19 different stress conditions. These mutants are characterized by a constitutively upregulated antioxidative defense system and, therefore, adaptation to oxidative stress may play an important role in gaining general stress tolerance in C. albicans. Although C. albicans cells may undergo morphological transitions under various stress treatments, this ability shows considerable stress-specific and strain-specific variability and, hence, it is independent of mounting stress cross protections.

• MeSH
• Antioxidants physiology   MeSH
• Candida albicans genetics   physiology   ultrastructure   MeSH
• Microscopy, Fluorescence MeSH
• Adaptation, Physiological genetics   physiology   MeSH
• Microscopy, Phase-Contrast MeSH
• Mutation genetics   physiology   MeSH
• Oxidative Stress genetics   physiology   MeSH
• Up-Regulation genetics   physiology   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Antioxidants MeSH

A chromate-tolerant mutant chr1-663T bearing a stable one-gene mutation and its parental strain 6chr(+) were used to investigate the background of Cr(VI) tolerance in the fission yeast Schizosaccharomyces pombe. The mutant chr1-663T displayed a significantly decreased specific glutathione reductase (GR) activity coded by the pgr1 (+) gene compared with its parental strain. Transformants of the mutant chr1-663T with a nonintegrative pUR18N vector expressing the pgr1 (+) gene exhibited the same Cr(VI) sensitivity and specific GR activity as their parental strain, demonstrating the importance of the GR-NADPH system in Cr(VI) tolerance. Transformants, nevertheless, exhibited an increased intracellular peroxide concentration, a decreased Cr(VI)-reducing and HO*-producing ability, which suggested an unbalanced oxidoreduction state of cells and partial complementation of the GR function. No mutation was found in the sequences of the pgr1 (+) and the pap1 (+) (transcriptional regulatory gene of GR) genes of the Cr(VI)-tolerant mutant by sequence analysis.

• MeSH
• Chromates metabolism   pharmacology   MeSH
• Down-Regulation * MeSH
• Drug Resistance, Fungal MeSH
• Glutathione Reductase genetics   metabolism   MeSH
• Mutation * MeSH
• Oxidation-Reduction MeSH
• Pancreatitis-Associated Proteins MeSH
• Schizosaccharomyces pombe Proteins genetics   metabolism   MeSH
• Schizosaccharomyces drug effects   enzymology   genetics   metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Chromates MeSH
• Glutathione Reductase MeSH
• Pancreatitis-Associated Proteins MeSH
• REG3A protein, human MeSH Browser
• Schizosaccharomyces pombe Proteins MeSH