Effects of decreased specific glutathione reductase activity in a chromate-tolerant mutant of Schizosaccharomyces pombe
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
- MeSH
- chromany metabolismus farmakologie MeSH
- down regulace * MeSH
- fungální léková rezistence MeSH
- glutathionreduktasa genetika metabolismus MeSH
- mutace * MeSH
- oxidace-redukce MeSH
- proteiny asociované s pankreatitidou MeSH
- Schizosaccharomyces pombe - proteiny genetika metabolismus MeSH
- Schizosaccharomyces účinky léků enzymologie genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chromany MeSH
- glutathionreduktasa MeSH
- proteiny asociované s pankreatitidou MeSH
- REG3A protein, human MeSH Prohlížeč
- Schizosaccharomyces pombe - proteiny MeSH
A chromate-tolerant mutant chr1-663T bearing a stable one-gene mutation and its parental strain 6chr(+) were used to investigate the background of Cr(VI) tolerance in the fission yeast Schizosaccharomyces pombe. The mutant chr1-663T displayed a significantly decreased specific glutathione reductase (GR) activity coded by the pgr1 (+) gene compared with its parental strain. Transformants of the mutant chr1-663T with a nonintegrative pUR18N vector expressing the pgr1 (+) gene exhibited the same Cr(VI) sensitivity and specific GR activity as their parental strain, demonstrating the importance of the GR-NADPH system in Cr(VI) tolerance. Transformants, nevertheless, exhibited an increased intracellular peroxide concentration, a decreased Cr(VI)-reducing and HO*-producing ability, which suggested an unbalanced oxidoreduction state of cells and partial complementation of the GR function. No mutation was found in the sequences of the pgr1 (+) and the pap1 (+) (transcriptional regulatory gene of GR) genes of the Cr(VI)-tolerant mutant by sequence analysis.
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