Brucellosis is one of the most important zoonoses worldwide, primarily affecting livestock but also posing a serious threat to public health. The major Brucella species are known to cause a feverish disease in humans with various clinical signs. These classical Brucella species are (re-)emerging, but also novel strains and species, some of them transmitted from rodents, can be associated with human infections. As a result of our review on rodent-borne brucellosis, we emphasise the need for more comprehensive surveillance of Brucella and especially Brucella microti in rodent populations and call for further research targeting the ecological persistence of rodent-associated Brucella species in the environment, their epizootic role in wild rodents and their virulence and pathogenicity for wildlife.

• Klíčová slova
• Brucella, public health risk, rodent, sapronosis, zoonosis,
• MeSH
• Brucella * izolace a purifikace   MeSH
• brucelóza * epidemiologie   veterinární   mikrobiologie   přenos   MeSH
• hlodavci * mikrobiologie   MeSH
• lidé MeSH
• veřejné zdravotnictví * MeSH
• zoonózy * MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH

The evolutionary "success" of the genus Brucella depends on the ability to persist both in the environment as well as inside of even activated macrophages of the animal host. For that, the Brucellae produce catalase and superoxide dismutase to defend against oxidative stress. Since the deletion of the mglA gene in the B. abortus S19 vaccine strain resulted not only in an increased tolerance to H2O2 but also in the induction of cytokines in macrophages, we here investigated the effect of oxidative stress (Fe2+ and H2O2) on the survival of B. abortus S19 and the isogenic B. abortus S 19 ∆mglA 3.14 deletion mutant in comparison with B. neotomae 5K33, Brucella strain 83/13, and B. microti CCM4915. These Brucellae belong to different phylogenetic clades and show characteristic differences in the mgl-operon. From the various Brucellae tested, B. abortus S19 showed the highest susceptibility to oxidative stress and the lowest ability to survive inside of murine macrophages. B. abortus S19 ∆mglA 3.14 as well as B. neotomae, which also belongs to the classical core clade of Brucella and lacks the regulators of the mgl-operon, presented the highest degree of tolerance to H2O2 but not in the survival in macrophages. The latter was most pronounced in case of an infection with B. 83/13 and B. microti CCM4915. The various Brucellae investigated here demonstrate significant differences in tolerance against oxidative stress and different survival in murine macrophages, which, however, do not correlate directly.

• Klíčová slova
• Brucella, Fe2 +, H2O2, Macrophages, Oxidative stress,
• MeSH
• adenosintrifosfát metabolismus   MeSH
• bakteriální geny MeSH
• Brucella abortus fyziologie   MeSH
• Brucella klasifikace   fyziologie   MeSH
• buněčné linie MeSH
• cytokiny metabolismus   MeSH
• druhová specificita MeSH
• makrofágy imunologie   mikrobiologie   MeSH
• mikrobiální viabilita MeSH
• mutace MeSH
• myši MeSH
• oxidační stres * MeSH
• peroxid vodíku metabolismus   MeSH
• počet mikrobiálních kolonií MeSH
• železo metabolismus   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• adenosintrifosfát MeSH
• cytokiny MeSH
• peroxid vodíku MeSH
• železo MeSH