The susceptibility toward antibiotics was determined by disc and MIC methods in Lactobacillus and, for comparison, in Escherichia coli strains isolated from cloacal swabs of broiler chickens derived from various farms in Slovakia. The occurrence of acquired tetracycline resistance in E. coli and lactobacilli isolated from the same sample was similar. The presence of tet(M), tet(S), tet(L) and ermB genes was demonstrated in lactobacilli while the tet(M) gene was not detected in E. coli.

• MeSH
• Anti-Bacterial Agents pharmacology   MeSH
• Genes, Bacterial MeSH
• Drug Resistance, Bacterial * MeSH
• Escherichia coli drug effects   isolation & purification   MeSH
• Cloaca microbiology   MeSH
• Chickens MeSH
• Lactobacillus drug effects   isolation & purification   MeSH
• Microbial Sensitivity Tests MeSH
• Polymerase Chain Reaction MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Slovakia MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH

Twelve lactobacilli isolates from mucosa of 3-5-week-old weaned pigs were found to exert good antimicrobial activity against common porcine pathogens (S. aureus, B. cereus, E. coli, C. perfringens). Two of them produced in addition to lactic acid also considerable amounts of acetic acid, and 6 of them produced hydrogen peroxide and metabolites other than organic acids. Isolates 4/26 and 2/25 (identified as L. crispatus or L. amylovorus) were inhibitory against most strains of S. aureus, B. cereus and E. coli, and especially the strain 4/26 survived well in simulated gastric and intestinal juice. Diarrhea-causing E. coli O8K88H9 Ent(+) was successfully inhibited by the growing culture as well as by the catalase-treated and neutralized supernatant of L. reuteri 12/26. Mucin degradation and multiple resistance to antibiotics were not observed.

• MeSH
• Drug Resistance, Microbial MeSH
• Bacillus cereus drug effects   MeSH
• Clostridium perfringens drug effects   MeSH
• Escherichia coli drug effects   MeSH
• Feces microbiology   MeSH
• Ileum microbiology   MeSH
• Culture Media, Conditioned pharmacology   MeSH
• Culture Media chemistry   pharmacology   MeSH
• Lactic Acid pharmacology   MeSH
• Lactobacillus drug effects   isolation & purification   metabolism   MeSH
• Mucins metabolism   MeSH
• Swine Diseases prevention & control   MeSH
• Weaning MeSH
• Hydrogen Peroxide metabolism   MeSH
• Immunity, Innate MeSH
• Probiotics MeSH
• Staphylococcus aureus drug effects   MeSH
• Intestinal Mucosa microbiology   MeSH
• Sus scrofa microbiology   MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Culture Media, Conditioned MeSH
• Culture Media MeSH
• Lactic Acid MeSH
• Mucins MeSH
• Hydrogen Peroxide MeSH