Eleven type strains and 24 Lactobacillus isolates, preliminarily classified to the species due to phenotypic features, were investigated. Standard methods of identification with species-specific PCRs and typing with PFGE (with ApaI, NotI and SmaI restriction enzymes) allowed us to distinguish 16 unique strains belonging to 5 species (L. acidophilus, L. delbrueckii ssp. bulgaricus, L. plantarum, L. rhamnosus, L. salivarius). Alternative approach with 16S-23S rDNA ARDRA identification (with merely two restrictases, BsuRI and TaqI) and PCR-based typing (RAPD with two random- and rep-PCR with (GTG)(5) primers) showed to be more discriminative, i.e. 21 unique strains were classified in the same species as above. As a result, 7 out of 24 phenotypically species-assigned isolates were reclassified. The alternative procedure of rapid identification and typing of Lactobacillus isolates appeared to be equally effective and shortened from 1 week to 2-3 d (in comparison to the standard methods).

• MeSH
• časové faktory MeSH
• DNA bakterií analýza   genetika   MeSH
• DNA primery MeSH
• druhová specificita MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• fenotyp MeSH
• Lactobacillus klasifikace   genetika   izolace a purifikace   MeSH
• polymerázová řetězová reakce metody   MeSH
• restrikční mapování metody   MeSH
• RNA ribozomální 16S genetika   MeSH
• RNA ribozomální 23S genetika   MeSH
• technika náhodné amplifikace polymorfní DNA metody   MeSH
• techniky typizace bakterií metody   MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• Názvy látek
• DNA bakterií MeSH
• DNA primery MeSH
• RNA ribozomální 16S MeSH
• RNA ribozomální 23S MeSH

The "cocktail" of human origin rifampicin-resistant bifidobacteria (RRBs) and RRBs from commercial products was administrated to 9 volunteers aged from 22 to 46 years and the survival ability in gastrointestinal tract of these strains was determined. Bifidobacteria represented 0-8 % of total anaerobes detected in gastrointestinal tract of volunteers before the administration of probiotic strains. After the administration of probiotics, bifidobacterial counts increased to 16 % of total bacterial counts. RRBs formed 9-44 % of total counts of bifidobacteria. Then, the counts of RRBs decreased at day 7 after administration, and they were not detected after 14 d. In our study, suitable probiotic bifidobacterial strains for human should be chosen on the basic of in vitro test but the results showed that no strain was able to colonize human tract permanently.

• MeSH
• Bifidobacterium fyziologie   MeSH
• dospělí MeSH
• experimenty na lidech MeSH
• gastrointestinální trakt mikrobiologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mikrobiální viabilita * MeSH
• počet mikrobiálních kolonií MeSH
• probiotika aplikace a dávkování   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH