Eleven type strains and 24 Lactobacillus isolates, preliminarily classified to the species due to phenotypic features, were investigated. Standard methods of identification with species-specific PCRs and typing with PFGE (with ApaI, NotI and SmaI restriction enzymes) allowed us to distinguish 16 unique strains belonging to 5 species (L. acidophilus, L. delbrueckii ssp. bulgaricus, L. plantarum, L. rhamnosus, L. salivarius). Alternative approach with 16S-23S rDNA ARDRA identification (with merely two restrictases, BsuRI and TaqI) and PCR-based typing (RAPD with two random- and rep-PCR with (GTG)(5) primers) showed to be more discriminative, i.e. 21 unique strains were classified in the same species as above. As a result, 7 out of 24 phenotypically species-assigned isolates were reclassified. The alternative procedure of rapid identification and typing of Lactobacillus isolates appeared to be equally effective and shortened from 1 week to 2-3 d (in comparison to the standard methods).

• MeSH
• Time Factors MeSH
• DNA, Bacterial analysis   genetics   MeSH
• DNA Primers MeSH
• Species Specificity MeSH
• Electrophoresis, Polyacrylamide Gel MeSH
• Phenotype MeSH
• Lactobacillus classification   genetics   isolation & purification   MeSH
• Polymerase Chain Reaction methods   MeSH
• Restriction Mapping methods   MeSH
• RNA, Ribosomal, 16S genetics   MeSH
• RNA, Ribosomal, 23S genetics   MeSH
• Random Amplified Polymorphic DNA Technique methods   MeSH
• Bacterial Typing Techniques methods   MeSH
• Publication type
• Journal Article MeSH
• Evaluation Study MeSH
• Names of Substances
• DNA, Bacterial MeSH
• DNA Primers MeSH
• RNA, Ribosomal, 16S MeSH
• RNA, Ribosomal, 23S MeSH

The "cocktail" of human origin rifampicin-resistant bifidobacteria (RRBs) and RRBs from commercial products was administrated to 9 volunteers aged from 22 to 46 years and the survival ability in gastrointestinal tract of these strains was determined. Bifidobacteria represented 0-8 % of total anaerobes detected in gastrointestinal tract of volunteers before the administration of probiotic strains. After the administration of probiotics, bifidobacterial counts increased to 16 % of total bacterial counts. RRBs formed 9-44 % of total counts of bifidobacteria. Then, the counts of RRBs decreased at day 7 after administration, and they were not detected after 14 d. In our study, suitable probiotic bifidobacterial strains for human should be chosen on the basic of in vitro test but the results showed that no strain was able to colonize human tract permanently.

• MeSH
• Bifidobacterium physiology   MeSH
• Adult MeSH
• Human Experimentation MeSH
• Gastrointestinal Tract microbiology   MeSH
• Middle Aged MeSH
• Humans MeSH
• Microbial Viability * MeSH
• Colony Count, Microbial MeSH
• Probiotics administration & dosage   MeSH
• Check Tag
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH